ArticleMolecular biology of the cell2004
Ectopic EphA4 receptor induces posterior protrusions via FGF signaling in Xenopus embryos.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Roles of Eph-Ephrin Signaling in the Eye Lens Cataractogenesis, Biomechanics, and Homeostasis.Frontiers in cell and developmental biology · 2022Review
- A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain.The Journal of biological chemistry · 2021Article
- A Novel Occulta-Type Spina Bifida Mediated by Murine Double HeterozygotesFrontiers in cell and developmental biology · 2017Article
- Variable combinations of specific ephrin ligand/Eph receptor pairs control embryonic tissue separation.PLoS biology · 2014Article
- The composition of EphB2 clusters determines the strength in the cellular repulsion response.The Journal of cell biology · 2014Article
- Roles of EphA2 in Development and Disease.Genes · 2013Article
- Global evaluation of Eph receptors and ephrins in lung adenocarcinomas identifies EphA4 as an inhibitor of cell migration and invasion.Molecular cancer therapeutics · 2012Article
- Eph-dependent cell-cell adhesion and segregation in development and cancer.Cellular and molecular life sciences : CMLS · 2012Review
- The role of Eph receptors in lens function and disease.Science China. Life sciences · 2012Review
- EphA receptor signaling--complexity and emerging themes.Seminars in cell & developmental biology · 2012Review
- NMR structure of a heterodimeric SAM:SAM complex: characterization and manipulation of EphA2 binding reveal new cellular functions of SHIP2.Structure (London, England : 1993) · 2012Article
- Article
- Eph/ephrin signaling in cell-cell and cell-substrate adhesion.Frontiers in bioscience (Landmark edition) · 2012Review
- Interaction of Sox1, Sox2, Sox3 and Oct4 during primary neurogenesis.Developmental biology · 2011Article
- Eph/ephrin signaling: networks.Genes & development · 2008Review
- ephrinB1 signals from the cell surface to the nucleus by recruitment of STAT3.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A change in conformational dynamics underlies the activation of Eph receptor tyrosine kinases.The EMBO journal · 2006Article
- Article
- Williams Syndrome Transcription Factor is critical for neural crest cell function in Xenopus laevis.Mechanisms of developmentArticle
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The Eph family of receptor tyrosine kinases regulates numerous biological processes. To examine the biochemical and developmental contributions of specific structural motifs within Eph receptors, wild-type or mutant forms of the EphA4 receptor were ectopically expressed in developing Xenopus embryos. Wild-type EphA4 and a mutant lacking both the SAM domain and PDZ binding motif were constitutively tyrosine phosphorylated in vivo and catalytically active in vitro. EphA4 induced loss of cell adhesion, ventro-lateral protrusions, and severely expanded posterior structures in Xenopus embryos. Moreover, mutation of a conserved SAM domain tyrosine to phenylalanine (Y928F) enhanced the ability of EphA4 to induce these phenotypes, suggesting that the SAM domain may negatively regulate some aspects of EphA4 activity in Xenopus. Analysis of double mutants revealed that the Y928F EphA4 phenotypes were dependent on kinase activity; juxtamembrane sites of tyrosine phosphorylation and SH2 domain-binding were required for cell dissociation, but not for posterior protrusions. The induction of protrusions and expansion of posterior structures is similar to phenotypic effects observed in Xenopus embryos expressing activated FGFR1. Furthermore, the budding ectopic protrusions induced by EphA4 express FGF-8, FGFR1, and FGFR4a. In addition, antisense morpholino oligonucleotide-mediated loss of FGF-8 expression in vivo substantially reduced the phenotypic effects in EphA4Y928F expressing embryos, suggesting a connection between Eph and FGF signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.