ArticleInfection and immunity2004
Molecular characterization of a glucose-inhibited division gene, gidA, that regulates cytotoxic enterotoxin of Aeromonas hydrophila.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 80 citations in OpenAlex.
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- A tRNA modifying enzyme as a tunable regulatory nexus for bacterial stress responses and virulence.Nucleic acids research · 2022Article
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- A transposon mutant library of Bacillus cereus ATCC 10987 reveals novel genes required for biofilm formation and implicates motility as an important factor for pellicle-biofilm formation.MicrobiologyOpen · 2018Article
- The Eukaryote-Like Serine/Threonine Kinase STK Regulates the Growth and Metabolism of ZoonoticFrontiers in cellular and infection microbiology · 2017Article
- GidA, a tRNA Modification Enzyme, Contributes to the Growth, and Virulence of Streptococcus suis Serotype 2.Frontiers in cellular and infection microbiology · 2016Article
- Virulence Factors of Aeromonas hydrophila: In the Wake of Reclassification.Frontiers in microbiology · 2016Review
- Novel insights into the pathogenicity of epidemic Aeromonas hydrophila ST251 clones from comparative genomics.Scientific reports · 2015Article
- Molecular characterization of protease activity in Serratia sp. strain SCBI and its importance in cytotoxicity and virulence.Journal of bacteriology · 2014Article
- tRNA modification enzymes GidA and MnmE: potential role in virulence of bacterial pathogens.International journal of molecular sciences · 2014Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
By using a mini-transposon, we obtained two mutated strains of a diarrheal isolate, SSU, of Aeromonas hydrophila that exhibited a 50 to 53% reduction in the hemolytic activity and 83 to 87% less cytotoxic activity associated with the cytotoxic enterotoxin (Act). Act is a potent virulence factor of A. hydrophila and has been shown to contribute significantly to the development of both diarrhea and septicemia in animal models. Subsequent cloning and DNA sequence analysis revealed that transposon insertion occurred at different locations in these two mutants within the same 1,890-bp open reading frame for the glucose-inhibited division gene (gidA). A similar reduction in hemolytic (46%) and cytotoxic (81%) activity of Act was noted in the gidA isogenic mutant of A. hydrophila that was generated by marker exchange mutagenesis. Northern blot analysis revealed that the transcription of the cytotoxic enterotoxin gene (act) was not altered in the gidA transposon and isogenic mutants. However, by generating a chromosomal act::alkaline phosphatase gene (phoA) reporter construct, we demonstrated significantly reduced phosphatase activity in these mutants, indicating the effect of glucose-inhibited division (GidA) protein in modulating act gene expression at the translational level. The biological effects of Act in the gidA mutants were restored by complementation. The virulence of the gidA mutants in mice was dramatically reduced compared to the those of the wild-type (WT) and complemented strains of A. hydrophila. The histopathological examination of lungs, in particular, indicated severe congestion, alveolar hemorrhage, and acute inflammatory infiltrate in the interstitial compartment and the alveolar spaces when mice were infected with the WT and complemented strains. Minimal-to-mild changes were noted in the lungs with the gidA mutants. Taken together, our data indicate for the first time that GidA regulates the most-potent virulence factor of A. hydrophila, Act.
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