ArticleInfection and immunity2004
The Cryptosporidium oocyst wall protein is a member of a multigene family and has a homolog in Toxoplasma.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 103 citations in OpenAlex.
- Insights into the lifecycle of Cryptosporidium and compounds targeting developmental stages.Current opinion in microbiology · 2026Review
- Cryptosporidium oocyst wall proteins are true components of the oocyst wall and COWP8 is not required for parasite transmission.PLoS pathogens · 2025Article
- Molecular detection ofOpen veterinary journal · 2024Article
- Cellulose binding and the timing of expression influence protein targeting to the double-layered cyst wall ofmSphere · 2024Article
- Article
- UnveilingFrontiers in cellular and infection microbiology · 2024Article
- First microscopic and molecular identification ofFrontiers in veterinary science · 2024Article
- Comparative proteomic analysis of wall-forming bodies and oocyst wall reveals the molecular basis underlying oocyst wall formation in Eimeria necatrix.Parasites & vectors · 2023Article
- Proteomic Analysis of FractionatedInternational journal of molecular sciences · 2023Article
- Enrichment and proteomic identification of Cryptosporidium parvum oocyst wall.Parasites & vectors · 2022Article
- Live imaging of the Cryptosporidium parvum life cycle reveals direct development of male and female gametes from type I meronts.PLoS biology · 2022Article
- Sexual Development in Non-Human Parasitic Apicomplexa: Just Biology or Targets for Control?Animals : an open access journal from MDPI · 2021Review
- Identification and Characterization ofMicrobiology spectrum · 2021Article
- Use of Oxidative Stress Responses to Determine the Efficacy of Inactivation Treatments onMicroorganisms · 2021Article
- In Silico Identification and Functional Characterization of Conserved miRNAs in the Genome ofBioinformatics and biology insights · 2021Article
- Polyphyletic origin, intracellular invasion, and meiotic genes in the putatively asexual agamococcidians (Apicomplexa incertae sedis).Scientific reports · 2020Article
- Characterization of Cystoisospora suis sexual stages in vitro.Parasites & vectors · 2020Article
- Review
- Comparisons of the Sexual Cycles for the Coccidian ParasitesFrontiers in cellular and infection microbiology · 2020Review
- Structure, composition, and roles of theCell surface (Amsterdam, Netherlands) · 2019Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Coccidian parasites are transmitted via a fecal oocyst stage that is exceptionally resistant to environmental stress and harsh chemical treatments, which allows parasites to stably persist outside a host. Because of its oocyst durability Cryptosporidium parvum is a significant water- and food-borne pathogen of humans, as well as animals of agricultural importance. To date, only one apicomplexan oocyst membrane protein has been identified, Cryptosporidium oocyst wall protein 1 (COWP1). COWP1 has a highly cysteine-rich periodicity due to arrays of two apicomplexan-specific motifs, designated the type I and type II domains. In this study, exhaustive BLAST screening of a complete C. parvum genome sequence database resulted in identification of eight additional genes encoding similar arrays of cysteine-rich type I and/or type II domains. Transcript expression analysis revealed that all COWP genes are abundantly expressed at a time when developing oocysts are observed, roughly 48 to 72 h after inoculation of in vitro cultures. A monoclonal antibody recognizing COWP8 specifically localized to the C. parvum oocyst wall, supporting the hypothesis that multiple COWPs play a role in the oocyst wall structure. BLAST screening of the Toxoplasma gondii genome sequence database resulted in identification of a gene encoding at least one COWP homolog (TgOWP1), and this multiexon sequence information was used to isolate a full-length cDNA. Exhaustive screening of Plasmodium sp. genome sequence databases by using COWP genes as BLAST queries failed to detect similar proteins in PLASMODIUM: We therefore propose that the COWP family of proteins have a structural role in apicomplexan species that produce durable shed cysts capable of surviving environmental stress.
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