ArticleInfection and immunity2004
Host-inducible immunogenic sphingomyelinase-like protein, Lk73.5, of Leptospira interrogans.
Article in Infection and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 53 citations in OpenAlex.
- Cultivation of Leptospira interrogans Within Rat Peritoneal Dialysis Membrane Chambers.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A clinical pilot study for the detection of sphingomyelinase in leptospirosis patient's urine at tertiary care hospital.Heliyon · 2023Article
- Sph2Vaccines · 2023Article
- Characterization of a Novel Peptide from PathogenicPathogens (Basel, Switzerland) · 2020Article
- Article
- Lipidomic analysis of immune activation in equine leptospirosis and Leptospira-vaccinated horses.PloS one · 2018Article
- Temperature and Oxidative Stress as Triggers for Virulence Gene Expression in PathogenicFrontiers in microbiology · 2017Article
- Role of sph2 Gene Regulation in Hemolytic and Sphingomyelinase Activities Produced by Leptospira interrogans.PLoS neglected tropical diseases · 2015Article
- A model system for studying the transcriptomic and physiological changes associated with mammalian host-adaptation by Leptospira interrogans serovar Copenhageni.PLoS pathogens · 2014Article
- Identification of Sphingomyelinase on the Surface of Chlamydia pneumoniae: Possible Role in the Entry into Its Host Cells.Interdisciplinary perspectives on infectious diseases · 2014Article
- Multiple leptospiral sphingomyelinases (or are there?).Microbiology (Reading, England) · 2012Review
- Methylation and in vivo expression of the surface-exposed Leptospira interrogans outer-membrane protein OmpL32.Microbiology (Reading, England) · 2012Article
- Development of transcriptional fusions to assess Leptospira interrogans promoter activity.PloS one · 2011Article
- Transcriptional responses of Leptospira interrogans to host innate immunity: significant changes in metabolism, oxygen tolerance, and outer membrane.PLoS neglected tropical diseases · 2010Article
- Leptospira as an emerging pathogen: a review of its biology, pathogenesis and host immune responses.Future microbiology · 2010Review
- Leptospiral endostatin-like protein A is a bacterial cell surface receptor for human plasminogen.Infection and immunity · 2010Article
- Evaluation of the expression and protective potential of Leptospiral sphingomyelinases.Current microbiology · 2010Article
- Comparative transcriptional and translational analysis of leptospiral outer membrane protein expression in response to temperature.PLoS neglected tropical diseases · 2009Article
- Global proteome analysis of Leptospira interrogans.Journal of proteome research · 2009Article
- LruA and LruB antibodies in sera of humans with leptospiral uveitis.Clinical and vaccine immunology : CVI · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leptospira interrogans causes a variety of clinical syndromes in animals and humans. Although much information has accumulated on the importance of leptospiral lipopolysaccharide in protective antibody responses, relatively little is known about proteins that participate in immune responses. Identification of those proteins induced only in the host is particularly difficult. Using a novel double-antibody screen designed to identify clones in a gene library of L. interrogans serovar Pomona expressing host-inducible proteins, we have characterized a gene (lk75.3) encoding a sphingomyelinase-like preprotein of 648 amino acids with cytotoxic activity for equine pulmonary endothelial cells and weak hemolytic activity for equine and rabbit erythrocytes. lk73.5 was found as a single gene copy in all serovars of L. interrogans but not in other Leptospira spp. except L. inadai. The open reading frame (ORF) for Lk73.5 is followed by another partially homologous sequence containing an ORF (sph-like 2) for a 28.7-kDa peptide. Lk73.5 and Sph-like 2 share 95.1 and 97.7% amino acid identity with putative sphingomyelinases Sph2 and Sph1 (N terminus) from L. interrogans serovar Lai (S.-X. Ren, G. Fu, X.-G. Jiangk, R. Zeng, Y.-G. Miao, H. Xu, Y.-X. Zhang, H. Xiong, G. Lu, L.-F. Lu, H.-Q. Jiang, J. Jia, Y.-F. Tu, J.-X. Jiang, W.-Y. Gu, Y.-Q. Zhang, Z. Cai, H.-H. Sheng, H.-F. Yin, Y. Zhang, G.-F. Zhu, M. Wank, H.-L. Huangk, Z. Qian, S.-Y. Wang, Wei Ma, Z.-J. Yao, Y. Shen, B.-Q. Qiang, Q.-C. Xia, X.-K. Guo, A. Danchinq, I. S. Girons, R. L. Somerville, Y.-M. Wen, M.-H. Shik, Z. Chen, J.-G. Xuk, and G.-P. Zhao, Nature 422:88-893, 2003). Substantial homologies to sphingomyelinases from other leptospiras and other bacteria are also present. Lk73.5 was not detected in leptospiras cultured at 30 or 37 degrees C. The recombinant protein reacted strongly with sera from recently infected mares but not with sera from horses vaccinated with commercial pentavalent bacterin. The host-inducible immunogenic Lk73.5 should have value in distinguishing vaccine from infection immune response.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.