ArticleThe EMBO journal2004
Crystal structures of histone Sin mutant nucleosomes reveal altered protein-DNA interactions.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed, 130 citations in OpenAlex.
- Histone Modifications in the Cell Cycle ofEpigenomes · 2026Review
- Cryo-EM structure and biochemical analyses of the nucleosome containing the cancer-associated histone H3 mutation E97K.Genes to cells : devoted to molecular & cellular mechanisms · 2024Article
- DNA Repair in Nucleosomes: Insights from Histone Modifications and Mutants.International journal of molecular sciences · 2024Review
- Histone 3.3-related chromatinopathy: missense variants throughout H3-3A and H3-3B cause a range of functional consequences across species.Human genetics · 2024Review
- Histone mutations in cancer.Biochemical Society transactions · 2023Article
- Micromechanical Study of Hyperacetylated Nucleosomes Using Single Molecule Transverse Magnetic Tweezers.International journal of molecular sciences · 2023Article
- TGM2-mediated histone transglutamination is dictated by steric accessibility.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Matching protein surface structural patches for high-resolution blind peptide docking.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- SMOG 2 and OpenSMOG: Extending the limits of structure-based models.Protein science : a publication of the Protein Society · 2022Article
- Nucleosomes and their complexes in the cryoEM era: Trends and limitations.Frontiers in molecular biosciences · 2022Article
- Structural studies of functional nucleosome complexes with transacting factors.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2022Review
- Surprising Twists in Nucleosomal DNA with Implication for Higher-order Folding.Journal of molecular biology · 2021Article
- Histone H2B Mutations in Cancer.Biomedicines · 2021Review
- NDF, a nucleosome-destabilizing factor that facilitates transcription through nucleosomes.Genes & development · 2018Article
- Structural rearrangements of the histone octamer translocate DNA.Nature communications · 2018Article
- Distortion of histone octamer core promotes nucleosome mobilization by a chromatin remodeler.Science (New York, N.Y.) · 2017Article
- Excess free histone H3 localizes to centrosomes for proteasome-mediated degradation during mitosis in metazoans.Cell cycle (Georgetown, Tex.) · 2016Article
- Article
- Nucleosome architecture throughout the cell cycle.Scientific reports · 2016Article
- In Vitro Chromatin Assembly: Strategies and Quality Control.Methods in enzymology · 2016Article
20 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Here we describe 11 crystal structures of nucleosome core particles containing individual point mutations in the structured regions of histones H3 and H4. The mutated residues are located at the two protein-DNA interfaces flanking the nucleosomal dyad. Five of the mutations partially restore the in vivo effects of SWI/SNF inactivation in yeast. We find that even nonconservative mutations of these residues (which exhibit a distinct phenotype in vivo) have only moderate effects on global nucleosome structure. Rather, local protein-DNA interactions are disrupted and weakened in a subtle and complex manner. The number of lost protein-DNA interactions correlates directly with an increased propensity of the histone octamer to reposition with respect to the DNA, and with an overall destabilization of the nucleosome. Thus, the disruption of only two to six of the approximately 120 direct histone-DNA interactions within the nucleosome has a pronounced effect on nucleosome mobility and stability. This has implications for our understanding of how these structures are made accessible to the transcription and replication machinery in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.