ArticleThe EMBO journal2004
Regulation of checkpoint kinases through dynamic interaction with Crb2.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 49 citations in OpenAlex.
- The DNA damage checkpoint: A tale from budding yeast.Frontiers in genetics · 2022Review
- Histone H3 lysine 36 methyltransferase mobilizes NER factors to regulate tolerance against alkylation damage in fission yeast.Nucleic acids research · 2018Article
- Inner nuclear membrane protein Lem2 facilitates Rad3-mediated checkpoint signaling under replication stress induced by nucleotide depletion in fission yeast.Cellular signalling · 2016Article
- Hyperactive Cdc2 kinase interferes with the response to broken replication forks by trapping S.pombe Crb2 in its mitotic T215 phosphorylated state.Nucleic acids research · 2014Article
- Article
- Regulatory motifs in Chk1.Cell cycle (Georgetown, Tex.) · 2013Article
- HMGA2 inhibits apoptosis through interaction with ATR-CHK1 signaling complex in human cancer cells.Neoplasia (New York, N.Y.) · 2013Article
- An essential function for the ATR-activation-domain (AAD) of TopBP1 in mouse development and cellular senescence.PLoS genetics · 2013Article
- Multiple phosphorylation of Rad9 by CDK is required for DNA damage checkpoint activation.Cell cycle (Georgetown, Tex.) · 2012Article
- Phosphorylation-dependent interactions between Crb2 and Chk1 are essential for DNA damage checkpoint.PLoS genetics · 2012Article
- The Rad4(TopBP1) ATR-activation domain functions in G1/S phase in a chromatin-dependent manner.PLoS genetics · 2012Article
- Eukaryotic DNA damage checkpoint activation in response to double-strand breaks.Cellular and molecular life sciences : CMLS · 2012Review
- Dpb11 coordinates Mec1 kinase activation with cell cycle-regulated Rad9 recruitment.The EMBO journal · 2011Article
- BRCT domains: easy as one, two, three.Cell cycle (Georgetown, Tex.) · 2011Review
- ATR autophosphorylation as a molecular switch for checkpoint activation.Molecular cell · 2011Article
- The phosphorylation network for efficient activation of the DNA replication checkpoint in fission yeast.The Journal of biological chemistry · 2011Article
- Mre11 nuclease activity and Ctp1 regulate Chk1 activation by Rad3ATR and Tel1ATM checkpoint kinases at double-strand breaks.Molecular and cellular biology · 2011Article
- Structure and function of the Rad9-binding region of the DNA-damage checkpoint adaptor TopBP1.Nucleic acids research · 2011Article
- Article
- A genetic screen for replication initiation defective (rid) mutants in Schizosaccharomyces pombe.Cell division · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
ATR/Rad3-like kinases promote the DNA damage checkpoint through regulating Chk1 that restrains the activation of cyclin-dependent kinases. In fission yeast, Crb2, a BRCT-domain protein that is similar to vertebrate 53BP1, plays a crucial role in establishing this checkpoint. We report here that Crb2 regulates DNA damage checkpoint through temporal and dynamic interactions with Rad3, Chk1 and replication factor Cut5. The active complex formation between Chk1 and Crb2 is regulated by Rad3 and became maximal during the checkpoint arrest. Chk1 activation seems to need two steps of interaction changes: the loss of Rad3-Chk1 and Rad3-Crb2 interactions, and the association between hyperphosphorylated forms of Chk1 and Crb2. Chk1 is the major checkpoint kinase for the arrest of DNA polymerase mutants. The in vitro assay of Chk1 showed that its activation requires the presence of Crb2 BRCT. Hyperphosphorylation of Crb2 is also dependent on its intact BRCT. Finally, we show direct interaction between Rad3 and Crb2, which is inhibitory to Rad3 activity. Hence, Crb2 is the first to interact with both Rad3 and Chk1 kinases.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.