ArticleGenes and immunity2004
Mining microarray data to identify transcription factors expressed in naïve resting but not activated T lymphocytes.
Article in Genes and immunity, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 17 citations in OpenAlex.
- STAT3-mediated suppression of the pre-induction silencer Ets-2 in activated T helper cells.Molecular biology reports · 2026Article
- Transcription factor Ets-2 regulates the expression of key lymphotropic factors.Molecular biology reports · 2020Article
- Distinctive variation in the U3R region of the 5' Long Terminal Repeat from diverse HIV-1 strains.PloS one · 2018Article
- Ets-2 Acts As a Transcriptional Repressor of the Human Immunodeficiency Virus Type 1 through Binding to a Repressor-Activator Target Sequence of 5'-LTR.Frontiers in immunology · 2017Article
- Transcription Factor Ets-2 Acts as a Preinduction Repressor of Interleukin-2 (IL-2) Transcription in Naive T Helper Lymphocytes.The Journal of biological chemistry · 2016Article
- Urinary MicroRNA Profiling Predicts the Development of Microalbuminuria in Patients with Type 1 Diabetes.Journal of clinical medicine · 2015Article
- Homocysteine induces inflammatory transcriptional signaling in monocytes.Frontiers in bioscience (Landmark edition) · 2013Article
- 3D chromatin conformation correlates with replication timing and is conserved in resting cells.Nucleic acids research · 2012Article
- Implementation of exon arrays: alternative splicing during T-cell proliferation as determined by whole genome analysis.BMC genomics · 2010Article
- Genome-wide analysis of immune activation in human T and B cells reveals distinct classes of alternatively spliced genes.PloS one · 2009Article
- CBF-1 promotes transcriptional silencing during the establishment of HIV-1 latency.The EMBO journal · 2007Article
- Involvement of HTLV-I Tax and CREB in aneuploidy: a bioinformatics approach.Retrovirology · 2006Article
- Transcriptional repressor DREAM regulates T-lymphocyte proliferation and cytokine gene expression.The EMBO journal · 2005Article
- Impact on genetic networks in human macrophages by a CCR5 strain of human immunodeficiency virus type 1.Journal of virology · 2004Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptional repressors controlling the expression of cytokine genes have been implicated in a variety of physiological and pathological phenomena. An unknown repressor that binds to the distal NFAT element of the interleukin-2 (IL-2) gene promoter in naive T-helper lymphocytes has been implicated in autoimmune phenomena and has emerged as a potentially important factor controlling the latency of HIV-1. The aim of this paper was the identification of this repressor. We resorted to public microarray databases looking for DNA-binding proteins that are present in naïve resting T cells but are downregulated when the cells are activated. A Bayesian data mining statistical analysis uncovered 25 candidate factors. Of the 25, NFAT4 and the oncogene ets-2 bind to the common motif AAGGAG found in the HIV-1 LTR and IL-2 probes. Ets-2 binding site contains the three G's that have been shown to be important for binding of the unknown factor; hence, we considered it the likeliest candidate. Electrophoretic mobility shift assays confirmed cross-reactivity between the unknown repressor and anti-ets-2 antibodies, and cotransfection experiments demonstrated the direct involvement of Ets-2 in silencing the IL-2 promoter. Designing experiments for transcription factor analysis using microarrays and Bayesian statistical methodologies provides a novel way toward elucidation of gene control networks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.