ReviewMolecular biotechnology2004
Specificity and performance of PCR detection assays for microbial pathogens.
Review in Molecular biotechnology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Catching the ElusiveBiotech (Basel (Switzerland)) · 2026Review
- Comprehensive virome analysis ofFrontiers in insect science · 2026Article
- Detection of Viruses with Oncogenic and Oncomodulatory Potential in Head and Neck Tumors-External Auricle.Biomedicines · 2025Article
- Development of a High-Resolution Melting Method for the Detection of Clarithromycin-ResistantAntibiotics (Basel, Switzerland) · 2024Article
- Universal Digital High-Resolution Melt Analysis for the Diagnosis of Bacteremia.The Journal of molecular diagnostics : JMD · 2024Article
- Article
- CRISPR-Cas-based techniques for pathogen detection: Retrospect, recent advances, and future perspectives.Journal of advanced research · 2023Review
- A novel approach for simultaneous detection of the most common food-borne pathogens by multiplex qPCR.Biomolecules & biomedicine · 2023Article
- Conventional and advanced detection techniques of foodborne pathogens: A comprehensive review.Heliyon · 2023Article
- Isothermal Nucleic Acid Amplification Technologies for the Detection of Equine Viral Pathogens.Animals : an open access journal from MDPI · 2021Review
- Estimating included animal species in mixed crude drugs derived from animals using massively parallel sequencing.Scientific reports · 2021Article
- Article
- Amplification of nonspecific products in quantitative polymerase chain reactions (qPCR).Biomolecular detection and quantification · 2017Article
- Genetic Diversity and Differentiation of Colletotrichum spp. Isolates Associated with Leguminosae Using Multigene Loci, RAPD and ISSR.The plant pathology journal · 2014Article
- Widespread occurrence of bacterial human virulence determinants in soil and freshwater environments.Applied and environmental microbiology · 2013Article
- Mathematical modeling of 16S ribosomal DNA amplification reveals optimal conditions for the interrogation of complex microbial communities with phylogenetic microarrays.Bioinformatics (Oxford, England) · 2011Article
- Optimizing the analysis of human intestinal microbiota with phylogenetic microarray.FEMS microbiology ecology · 2011Article
- Quantitative detection of Schistosoma japonicum cercariae in water by real-time PCR.PLoS neglected tropical diseases · 2008Article
- MdACO expression during abscission: the use of 33P labeled primers in transcript quantitation.Molecular biotechnology · 2007Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR has become a widely used tool for detection, identification and differentiation of pathogenic microorganisms in diagnosis of animal and human diseases. However, quite a number of currently used protocols can be further optimized to exclude nonspecific reactions. On the one hand, target sequences as defined by primer binding sites should be checked carefully for the absence of significant homologies to other organisms in order to insure high specificity of detection. A major part of PCR assays is still based on target sequences in the ribosomal RNA operon, but, as the differentiating potential of this region is limited, genes encoding cellular proteins, such as toxins, surface antigens or enzymes, have been shown to be a viable alternative in many instances. On the other hand, various approaches are available to improve the performance of the amplification reaction itself. The kinetics of amplification is known to be heavily dependent on primer-to-template ratio, efficiency of primer annealing and enzyme-to-template ratio. In the present paper, recently published PCR detection assays for microorganisms, particularly bacterial pathogens, are reviewed and optimization strategies are explained. The practical implications and epidemiological consequences of routine use of PCR in the diagnostic laboratory are also discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.