ArticlePlant physiology2004
A transcriptomic and proteomic characterization of the Arabidopsis mitochondrial protein import apparatus and its response to mitochondrial dysfunction.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Knockout of mitochondrial dicarboxylate carriers AtDIC1 and AtDIC3 affects germination, biomass accumulation, and carbon partitioning in Arabidopsis thaliana.Plant cell reports · 2026Article
- Tric proteins and TOM complex subunits are involved in the import of short DNA fragments into Arabidopsis mitochondria.Protoplasma · 2025Article
- Uracil-DNA Glycosylase fromInternational journal of molecular sciences · 2025Article
- Imaging phenotype reveals that disulfirams induce protein insolubility in the mitochondrial matrix.Scientific reports · 2024Article
- Haplotype-resolved genome assembly and resequencing analysis provide insights into genome evolution and allelic imbalance in Pinus densiflora.Nature genetics · 2024Article
- Metatranscriptomics captures dynamic shifts in mycorrhizal coordination in boreal forests.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- High-resolution genetic mapping of a novel bacterial blight resistance gene xa-45(t) identified from Oryza glaberrima and transferred to Oryza sativa.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- The Arabidopsis Proteins AtNHR2A and AtNHR2B Are Multi-Functional Proteins Integrating Plant Immunity With Other Biological Processes.Frontiers in plant science · 2020Article
- Electrical Signaling, Photosynthesis and Systemic Acquired Acclimation.Frontiers in physiology · 2017Review
- Dissecting the Metabolic Role of Mitochondria during Developmental Leaf Senescence.Plant physiology · 2016Article
- Comparative transcript profiling of alloplasmic male-sterile lines revealed altered gene expression related to pollen development in rice (Oryza sativa L.).BMC plant biology · 2016Article
- Article
- Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency.BMC genomics · 2015Article
- Genome-wide analysis of adaptive molecular evolution in the carnivorous plant Utricularia gibba.Genome biology and evolution · 2015Article
- A simple method for the addition of rotenone in Arabidopsis thaliana leaves.Plant signaling & behavior · 2015Article
- TRANSLOCASE OF THE INNER MEMBRANE9 and 10 are essential for maintaining mitochondrial function during early embryo cell and endosperm free nucleus divisions in Arabidopsis.Plant physiology · 2014Article
- Evidence for interactions between the mitochondrial import apparatus and respiratory chain complexes via Tim21-like proteins in Arabidopsis.Frontiers in plant science · 2014Article
- Rate of alternative electron transport in arabidopsis mitochondria affects the expression of the glutamate dehydrogenase gene gdh2.Doklady. Biochemistry and biophysics · 2013Article
- AtWRKY40 and AtWRKY63 modulate the expression of stress-responsive nuclear genes encoding mitochondrial and chloroplast proteins.Plant physiology · 2013Article
- Transcriptome sequencing of the Antarctic vascular plant Deschampsia antarctica Desv. under abiotic stress.Planta · 2013Article
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Authors and funding
8 authors.
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Abstract
Mitochondria import hundreds of cytosolically synthesized proteins via the mitochondrial protein import apparatus. Expression analysis in various organs of 19 components of the Arabidopsis mitochondrial protein import apparatus encoded by 31 genes showed that although many were present in small multigene families, often only one member was prominently expressed. This was supported by comparison of real-time reverse transcriptase-polymerase chain reaction and microarray experimental data with expressed sequence tag numbers and massive parallel signature sequence data. Mass spectrometric analysis of purified mitochondria identified 17 import components, their mitochondrial sub-compartment, and verified the presence of TIM8, TIM13, TIM17, TIM23, TIM44, TIM50, and METAXIN proteins for the first time, to our knowledge. Mass spectrometry-detected isoforms correlated with the most abundant gene transcript measured by expression data. Treatment of Arabidopsis cell culture with mitochondrial electron transport chain inhibitors rotenone and antimycin A resulted in a significant increase in transcript levels of import components, with a greater increase observed for the minor isoforms. The increase was observed 12 h after treatment, indicating that it was likely a secondary response. Microarray analysis of rotenone-treated cells indicated the up-regulation of gene sets involved in mitochondrial chaperone activity, protein degradation, respiratory chain assembly, and division. The rate of protein import into isolated mitochondria from rotenone-treated cells was halved, even though rotenone had no direct effect on protein import when added to mitochondria isolated from untreated cells. These findings suggest that transcription of import component genes is induced when mitochondrial function is limited and that minor gene isoforms display a greater response than the predominant isoforms.
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