ArticlePlant physiology2004
Characterization and functional identification of a novel plant 4,5-extradiol dioxygenase involved in betalain pigment biosynthesis in Portulaca grandiflora.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 185 citations in OpenAlex.
- A cyclo-DOPA 6-O-glucosyltransferase-mediated route for gomphrenin I biosynthesis in Basella alba and Gomphrena globosa.The New phytologist · 2026Article
- Advances in Betalain Biosynthesis and Metabolic Engineering for Sustainable Natural Pigment Production.Biotech (Basel (Switzerland)) · 2026Review
- IbMYB1-4 positively regulates purple stem and influences leaf color via dosage effect of gene expression in sweetpotato (Ipomoea batatas (L.) Lam.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Article
- Flower color variations are determined by betalain biosynthetic genes in Portulaca umbraticola.Plant & cell physiology · 2025Article
- Multiple transitions to high l-DOPA 4,5-dioxygenase activity reveal molecular pathways to convergent betalain pigmentation in Caryophyllales.The New phytologist · 2025Article
- Identification ofPlant biotechnology (Tokyo, Japan) · 2025Article
- Structural Insights into 4,5-DOPA Extradiol Dioxygenase fromJournal of agricultural and food chemistry · 2025Article
- Differential Expression of Amaranth AtrDODA Gene Family Members in Betalain Synthesis and Functional Analysis of AtrDODA1-1 Promoter.Plants (Basel, Switzerland) · 2025Article
- Natural pigments derived from plants and microorganisms: classification, biosynthesis, and applications.Plant biotechnology journal · 2025Review
- BAHD acyltransferase from dragon fruit enables production of phyllocactin in engineered yeast.FEMS yeast research · 2025Article
- In planta complementation of the betalain biosynthetic pathway with a bacterial dioxygenase.PloS one · 2025Article
- High-efficiency production of plant-derived pigment dopaxanthin in Escherichia coli by combination engineering.Microbial cell factories · 2024Article
- Sustainable ultrasonic extraction of antibacterial Basella alba fruit dye for cotton, silk, and leather.Ultrasonics sonochemistry · 2024Article
- Comparison of the Intestinal Bacterial Communities between Captive and Semi-Free-Range Red-Crowned Cranes (Animals : an open access journal from MDPI · 2023Article
- Research Progress on Anthocyanin-Mediated Regulation of 'Black' Phenotypes of Plant Organs.Current issues in molecular biology · 2023Review
- Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot.BMC plant biology · 2023Article
- Combinatorial engineering of betalain biosynthesis pathway in yeast Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- Comparative transcriptome analysis of Zea mays upon mechanical wounding.Molecular biology reports · 2023Article
- Generating colorful carrot germplasm through metabolic engineering of betalains pigments.Horticulture research · 2023Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Betalains are pigments that replace anthocyanins in the majority of families of the plant order Caryophyllales. Betalamic acid is the common chromophore of betalains. The key enzyme of the betalain biosynthetic pathway is an extradiol dioxygenase that opens the cyclic ring of dihydroxy-phenylalanine (DOPA) between carbons 4 and 5, thus producing an unstable seco-DOPA that rearranges nonenzymatically to betalamic acid. A gene for a 4,5-DOPA-dioxygenase has already been isolated from the fungus Amanita muscaria, but no homolog was ever found in plants. To identify the plant gene, we constructed subtractive libraries between different colored phenotypes of isogenic lines of Portulaca grandiflora (Portulacaceae) and between different stages of flower bud formation. Using in silico analysis of differentially expressed cDNAs, we identified a candidate showing strong homology at the level of translated protein with the LigB domain present in several bacterial extradiol 4,5-dioxygenases. The gene was expressed only in colored flower petals. The function of this gene in the betalain biosynthetic pathway was confirmed by biolistic genetic complementation in white petals of P. grandiflora genotypes lacking the gene for color formation. This gene named DODA is the first characterized member of a novel family of plant dioxygenases phylogenetically distinct from Amanita sp. DOPA-dioxygenase. Homologs of DODA are present not only in betalain-producing plants but also, albeit with some changes near the catalytic site, in other angiosperms and in the bryophyte Physcomitrella patens. These homologs are part of a novel conserved plant gene family probably involved in aromatic compound metabolism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.