ArticleJournal of bacteriology2004
Binding of SycH chaperone to YscM1 and YscM2 activates effector yop expression in Yersinia enterocolitica.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 54 citations in OpenAlex.
- Type III secretion system chaperones: a helping hand for secretion.Microbiology (Reading, England) · 2026Review
- Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Uncovering Transient Protein Interactions in the Yersinia enterocolitica Type III Secretion System Using MiniTurbo-Mediated Proximity Labeling.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- Article
- Type III secretion systems: the bacterial flagellum and the injectisome.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- LcrQ blocks the role of LcrF in regulating the Ysc-Yop type III secretion genes in Yersinia pseudotuberculosis.PloS one · 2014Article
- Translational regulation of Yersinia enterocolitica mRNA encoding a type III secretion substrate.The Journal of biological chemistry · 2013Article
- YopK controls both rate and fidelity of Yop translocation.Molecular microbiology · 2013Article
- Identification of novel protein-protein interactions of Yersinia pestis type III secretion system by yeast two hybrid system.PloS one · 2013Article
- Protein export according to schedule: architecture, assembly, and regulation of type III secretion systems from plant- and animal-pathogenic bacteria.Microbiology and molecular biology reviews : MMBR · 2012Review
- Expression hierarchy in the Yersinia type III secretion system established through YopD recognition of RNA.Molecular microbiology · 2011Article
- YopK regulates the Yersinia pestis type III secretion system from within host cells.Molecular microbiology · 2011Article
- Cross-talk between type three secretion system and metabolism in Yersinia.The Journal of biological chemistry · 2009Article
- YtxR acts as an overriding transcriptional off switch for the Yersinia enterocolitica Ysc-Yop type 3 secretion system.Journal of bacteriology · 2009Article
- Secretion signal recognition by YscN, the Yersinia type III secretion ATPase.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Altered Ca(2+) regulation of Yop secretion in Yersinia enterocolitica after DNA adenine methyltransferase overproduction is mediated by Clp-dependent degradation of LcrG.Journal of bacteriology · 2006Article
- Staphylococcus aureus mutants with increased lysostaphin resistance.Journal of bacteriology · 2006Article
- Rejection of impassable substrates by Yersinia type III secretion machines.Journal of bacteriology · 2005Article
- Pseudomonas syringae type III chaperones ShcO1, ShcS1, and ShcS2 facilitate translocation of their cognate effectors and can substitute for each other in the secretion of HopO1-1.Journal of bacteriology · 2005Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Yersinia enterocolitica transports YscM1 and YscM2 via the type III pathway, a mechanism that is required for the establishment of bacterial infections. Prior to host cell contact, YscM1 and YscM2 exert posttranscriptional regulation to inhibit expression of effector yop genes, which encode virulence factors that travel the type III pathway into the cytoplasm of macrophages. Relief from repression has been predicted to occur via the type III secretion of YscM1 and YscM2 into the extracellular medium, resulting in the depletion of regulatory molecules from the bacterial cytoplasm. Using digitonin fractionation and fluorescence microscopy of FlAsH-labeled polypeptides in Yersinia-infected cells, we have localized YscM1 and YscM2 within the host cell cytoplasm. Type III injection of YscM1 and YscM2 required the SycH chaperone. Expression of C-terminal fusions of YscM1 and YscM2 to the neomycin phosphotransferase reporter revealed sequences required for regulatory activity and for secretion in the absence of SycH. Coexpression of SycH and glutathione S-transferase (GST)-YscM1 or GST-YscM2, hybrid GST variants that cannot be transported by the type III apparatus, also relieved repression of Yop synthesis. GST-SycH bound to YscM1 and YscM2 and activated effector yop expression without initiation of the bound regulatory molecules into the type III pathway. Further, regulation of yop expression by YscM1, YscM2, and SycH is shown to act independently of factors that regulate secretion, and gel filtration chromotography revealed populations of YscM1 and YscM2 that are not bound to SycH under conditions where Yop synthesis is repressed. Taken together, these results suggest that YscM1- and YscM2-mediated repression may be relieved through binding to the cytoplasmic chaperone SycH prior to their type III injection into host cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.