ArticleThe Journal of membrane biology2003
Characterization of a functionally expressed stretch-activated BKca channel cloned from chick ventricular myocytes.
Article in The Journal of membrane biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 49 citations in OpenAlex.
- Mechanotransduction-Driven Modulation ofInternational journal of molecular sciences · 2025Article
- The mechanism of CaThe Journal of biological chemistry · 2025Article
- Identification of RNA reads encoding different channels in isolated rat ventricular myocytes and the effect of cell stretching on L-type CaBiology direct · 2023Article
- Inhibition of BKCell death discovery · 2022Article
- Mechanosensitivity in Pulmonary Circulation: Pathophysiological Relevance of Stretch-Activated Channels in Pulmonary Hypertension.Biomolecules · 2021Review
- BKAntioxidants (Basel, Switzerland) · 2020Review
- The neuropeptide GsMTx4 inhibits a mechanosensitive BK channel through the voltage-dependent modification specific to mechano-gating.The Journal of biological chemistry · 2019Article
- Mechanosensitivity of the BK Channels in Human Glioblastoma Cells: Kinetics and Dynamical Complexity.The Journal of membrane biology · 2018Article
- Piezo channels and GsMTx4: Two milestones in our understanding of excitatory mechanosensitive channels and their role in pathology.Progress in biophysics and molecular biology · 2017Review
- Oxidative Stress and Maxi Calcium-Activated Potassium (BK) Channels.Biomolecules · 2015Review
- BK channels regulate sinoatrial node firing rate and cardiac pacing in vivo.American journal of physiology. Heart and circulatory physiology · 2014Article
- Effects of aerobic exercise training on large-conductance Ca(2+)-activated K (+) channels in rat cerebral artery smooth muscle cells.European journal of applied physiology · 2013Article
- Mechanobiology in cardiac physiology and diseases.Journal of cellular and molecular medicine · 2013Review
- Emerging Families of Ion Channels Involved in Urinary Bladder Nociception.Pharmaceuticals (Basel, Switzerland) · 2010Review
- Phosphatidylinositol 4,5-bisphosphate activates Slo3 currents and its hydrolysis underlies the epidermal growth factor-induced current inhibition.The Journal of biological chemistry · 2010Article
- Canonical transient receptor potential channel (TRPC)3 and TRPC6 associate with large-conductance Ca2+-activated K+ (BKCa) channels: role in BKCa trafficking to the surface of cultured podocytes.Molecular pharmacology · 2009Article
- Mechanosensitive ion channels and the peptide inhibitor GsMTx-4: history, properties, mechanisms and pharmacology.Toxicon : official journal of the International Society on Toxinology · 2007Review
- Twenty odd years of stretch-sensitive channels.Pflugers Archiv : European journal of physiology · 2006Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized electrophysiological and pharmacological properties of a stretch-activated BKca channel (SAKcaC) that was cloned from cultured chick ventricular myocytes (CCVM) and expressed in chinese hamster ovary cells (CHO) using the patch-clamp technique. Our results indicate that the cloned SAKcaC keeps most of the key properties of the native SAKcaC in CCVM, such as conductance, ion selectivity, pressure-, voltage- and Ca(2+)-dependencies. However, there was a slight difference between these channels in the effects of channel blockers, charybdotoxin (CTX) and gadolinium (Gd(3+)). The native SAKcaC was blocked in an all-or-none fashion characterized as the slow blockade, whereas the conductance of the cloned SAKcaC was gradually decreased with the blockers' concentration, without noticeable blocking noise. As the involvement of some auxiliary components was suspected in this difference, we cloned a BK beta-subunit from CCVM and coexpressed it with the cloned SAKcaC in CHO cells to examine its effects on the SAKcaC. Although the pharmacological properties of the cloned SAKcaC turned out to be very similar to the native one by the coexpression, it also significantly altered the key characteristics of SAKcaC, such as voltage- and Ca(2+)-dependencies. Therefore we concluded that the native SAKca in CCVM does not interact with the corresponding endogenous beta-subunit. The difference in pharmacological properties between the expressed SAKcaC in CHO and the native one in CCVM suggests that the native SAKca in CCVM is modulated by unknown auxiliary components.
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