ArticleThe Journal of membrane biology2003
Endothelial cell swelling by aldosterone.
Article in The Journal of membrane biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 98 citations in OpenAlex.
- Primary human neutrophils and monocytes migrate along endothelial cell boundaries to optimize search efficiency under static in vitro conditions.Biology open · 2025Article
- Endothelial cell serum and glucocorticoid regulated kinase 1 (SGK1) mediates vascular stiffening.Metabolism: clinical and experimental · 2024Article
- Endothelial sodium channel activation mediates DOCA-salt-induced endothelial cell and arterial stiffening.Metabolism: clinical and experimental · 2022Article
- ENaC in Salt-Sensitive Hypertension: Kidney and Beyond.Current hypertension reports · 2020Review
- The Epithelial Sodium Channel and the Processes of Wound Healing.BioMed research international · 2016Review
- AMP-Activated Protein Kinase Attenuates High Salt-Induced Activation of Epithelial Sodium Channels (ENaC) in Human Umbilical Vein Endothelial Cells.Oxidative medicine and cellular longevity · 2016Article
- Salt controls endothelial and vascular phenotype.Pflugers Archiv : European journal of physiology · 2015Review
- The role of ENaC in vascular endothelium.Pflugers Archiv : European journal of physiology · 2014Review
- Nanomechanics of vascular endothelium.Cell and tissue research · 2014Review
- Inhibition of mitochondrial aconitase by succination in fumarate hydratase deficiency.Cell reports · 2013Article
- High-speed atomic force microscopy combined with inverted optical microscopy for studying cellular events.Scientific reports · 2013Article
- Elasticity changes anti-correlate with NO production for human endothelial cells stimulated with TNF-α.Pflugers Archiv : European journal of physiology · 2012Article
- Adhesion and stress relaxation forces between melanoma and cerebral endothelial cells.European biophysics journal : EBJ · 2012Article
- Article
- Nitric oxide release follows endothelial nanomechanics and not vice versa.Pflugers Archiv : European journal of physiology · 2010Article
- Aldosterone receptor sites on plasma membrane of human vascular endothelium detected by a mechanical nanosensor.Pflugers Archiv : European journal of physiology · 2009Article
- Effect of excessive salt intake: role of plasma sodium.Current hypertension reports · 2009Review
- Therapeutic manipulation of glucocorticoid metabolism in cardiovascular disease.British journal of pharmacology · 2009Review
- Aldosterone and amiloride alter ENaC abundance in vascular endothelium.Pflugers Archiv : European journal of physiology · 2008Article
- Underexpression of mineralocorticoid receptor in colorectal carcinomas and association with VEGFR-2 overexpression.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2007Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is accumulating evidence that mineralocorticoids not only act on kidney but also on the cardiovascular system. We investigated the response of human umbilical venous endothelial cells (HUVECs) to aldosterone at a time scale of 20 minutes in absence and presence of the aldosterone antagonist spironolactone or other transport inhibitors. We applied atomic force microscopy (AFM), which measures cell volume and volume shifts between cytosol and cell nucleus. We observed an immediate cell volume increase (about 10%) approximately 1 min after addition of aldosterone (0.1 micromol/l), approaching a maximum (about 18%) 10 min after aldosterone treatment. Cell volume returned to normal 20 min after hormone exposure. Spironolactone (1 micromol/l) or amiloride (1 micromol/l) prevented the late aldosterone-induced volume changes but not the immediate change observed 1 min after hormone exposure. AFM revealed nuclear swelling 5 min after aldosterone addition, followed by nuclear shrinkage 15 min later. The Na(+)/H(+) exchange blocker cariporide (10 micromol/l) was ineffective. We conclude: (i). Aldosterone induces immediate (1 min) swelling independently of plasma membrane Na(+) channels and intracellular mineralocorticoid receptors followed by late mineralocorticoid receptor- and Na(+)-channel-dependent swelling. (ii). Intracellular macromolecule shifts cause the changes in cell volume. (iii). Both amiloride and spironolactone may be useful for medical applications to prevent aldosterone-induced vasculopathies.
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Registered trials
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