ArticleProceedings of the National Academy of Sciences of the United States of America2004
Calcium influx through hyperpolarization-activated cation channels (I(h) channels) contributes to activity-evoked neuronal secretion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Article
- Synthetic Cation Transporters Eradicate Drug-ResistantJACS Au · 2025Article
- Missing Puzzle Pieces in Dementia Research: HCN Channels and Theta Oscillations.Aging and disease · 2024Review
- Vascular mechanotransduction.Physiological reviews · 2023Review
- Purkinje cell dopaminergic inputs to astrocytes regulate cerebellar-dependent behavior.Nature communications · 2023Article
- Repurposing hyperpolarization-activated cyclic nucleotide-gated channels as a novel therapy for breast cancer.Clinical and translational medicine · 2021Article
- Oligodendrocyte HCN2 Channels Regulate Myelin Sheath Length.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- [Ru(bpy)Frontiers in pharmacology · 2019Article
- HCN Channels: New Therapeutic Targets for Pain Treatment.Molecules (Basel, Switzerland) · 2018Review
- Stretch-induced CaThe Journal of physiology · 2018Article
- Article
- Hyperpolarization-Activated Cyclic Nucleotide-Gated Non-selective (HCN) Ion Channels Regulate Human and Murine Urinary Bladder Contractility.Frontiers in physiology · 2018Article
- Potassium as a pluripotency-associated element identified through inorganic element profiling in human pluripotent stem cells.Scientific reports · 2017Article
- Intracellular TRPA1 mediates Ca2+ release from lysosomes in dorsal root ganglion neurons.The Journal of cell biology · 2016Article
- ZD7288, a selective hyperpolarization-activated cyclic nucleotide-gated channel blocker, inhibits hippocampal synaptic plasticity.Neural regeneration research · 2016Article
- From damage response to action potentials: early evolution of neural and contractile modules in stem eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- HCN Channels Modulators: The Need for Selectivity.Current topics in medicinal chemistry · 2016Review
- Stress Impairs Prefrontal Cortical Function via D1 Dopamine Receptor Interactions With Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels.Biological psychiatry · 2015Article
- Different downstream signalling of CCK1 receptors regulates distinct functions of CCK in pancreatic beta cells.British journal of pharmacology · 2015Article
- Bioelectric memory: modeling resting potential bistability in amphibian embryos and mammalian cells.Theoretical biology & medical modelling · 2015Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The hyperpolarization-activated cation channels (I(h)) play a distinct role in rhythmic activities in a variety of tissues, including neurons and cardiac cells. In the present study, we investigated whether Ca(2+) can permeate through the hyperpolarization-activated pacemaker channels (HCN) expressed in HEK293 cells and I(h) channels in dorsal root ganglion (DRG) neurons. Using combined measurements of whole-cell currents and fura-2 Ca(2+) imaging, we found that there is a Ca(2+) influx in proportion to I(h) induced by hyperpolarization in HEK293 cells. The I(h) channel blockers Cs(+) and ZD7288 inhibit both HCN current and Ca(2+) influx. Measurements of the fractional Ca(2+) current showed that it constitutes 0.60 +/- 0.02% of the net inward current through HCN4 at -120 mV. This fractional current is similar to that of the low Ca(2+)-permeable AMPA-R (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor) channels in Purkinje neurons. In DRG neurons, activation of I(h) for 30 s also resulted in a Ca(2+) influx and an elevated action potential-induced secretion, as assayed by the increase in membrane capacitance. These results suggest a functional significance for I(h) channels in modulating neuronal secretion by permitting Ca(2+) influx at negative membrane potentials.
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