ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2004
The effects of vesicular volume on secretion through the fusion pore in exocytotic release from PC12 cells.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed, 132 citations in OpenAlex.
- Profiling Endogenous Opioid Peptide Release from Adrenal Chromaffin Cells.ACS chemical neuroscience · 2026Article
- Analytical Determination of Serotonin Exocytosis in Human Platelets with BDD-on-Quartz MEA Devices.Biosensors · 2024Article
- Article
- Exploiting Microelectrode Geometry for Comprehensive Detection of Individual Exocytosis Events at Single Cells.ACS sensors · 2023Article
- Methodologies for Detecting Quantal Exocytosis in Adrenal Chromaffin Cells Through Diamond-Based MEAs.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Neurotransmitter Readily Escapes Detection at the Opposing Microelectrode Surface in Typical Amperometric Measurements of Exocytosis at Single Cells.Analytical chemistry · 2022Article
- Article
- Carbon-Fiber Nanoelectrodes for Real-Time Discrimination of Vesicle Cargo in the Native Cellular Environment.ACS nano · 2020Article
- The physiological role of α-synuclein and its relationship to Parkinson's Disease.Journal of neurochemistry · 2019Review
- Electrodeposited Gold on Carbon-Fiber Microelectrodes for Enhancing Amperometric Detection of Dopamine Release from Pheochromocytoma Cells.Analytical chemistry · 2018Article
- Fast-Scan Cyclic Voltammetry: Chemical Sensing in the Brain and Beyond.Analytical chemistry · 2018Review
- Vesicle impact electrochemical cytometry compared to amperometric exocytosis measurements.Current opinion in electrochemistry · 2017Article
- Mechanics of post-fusion exocytotic vesicle.Physical biology · 2017Article
- The F-Actin Binding Protein Cortactin Regulates the Dynamics of the Exocytotic Fusion Pore through its SH3 Domain.Frontiers in cellular neuroscience · 2017Article
- Exocytosis, Endocytosis, and Their Coupling in Excitable Cells.Frontiers in molecular neuroscience · 2017Review
- Single cell amperometry reveals curcuminoids modulate the release of neurotransmitters during exocytosis from PC12 cells.Journal of electroanalytical chemistry (Lausanne, Switzerland) · 2016Article
- ATP: The crucial component of secretory vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Cholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores.Biophysical journal · 2016Article
- Fast-scan cyclic voltammetry demonstrates that L-DOPA produces dose-dependent, regionally selective bimodal effects on striatal dopamine kinetics in vivo.Journal of neurochemistry · 2016Article
- Spatial resolution of single-cell exocytosis by microwell-based individually addressable thin film ultramicroelectrode arrays.Analytical chemistry · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many spikes in amperometric records of exocytosis events initially exhibit a prespike feature, or foot, which represents a steady-state flux of neurotransmitter through a stable fusion pore spanning both the vesicle and plasma membranes and connecting the vesicle lumen to the extracellular fluid. Here, we present the first evidence indicating that vesicular volume before secretion is strongly correlated with the characteristics of amperometric foot events. L-3,4-dihydroxyphenylalanine and reserpine have been used to increase and decrease, respectively, the volume of single pheochromocytoma cell vesicles. Amperometry and transmission electron microscopy have been used to determine that as vesicle size is decreased the frequency with which foot events are observed increases, the amount and duration of neurotransmitter released in the foot portion of the event decreases, and vesicles release a greater percentage of their total contents in the foot portion of the event. This previously unidentified correlation provides new insight into how vesicle volume can modulate the activity of the exocytotic fusion pore.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.