ArticleInternational journal of legal medicine2004
Development of a 13-locus PCR multiplex system for paternity testing.
Article in International journal of legal medicine, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Disputed paternity presumption in Burkina Faso: determination of the biological fathers of children using ABO-rhesus/hemoglobin electrophoresis and STR assays.Journal, genetic engineering & biotechnology · 2021Article
- Development and validation of a multiplex reaction analyzing eight miniSTRs of the X chromosome for identity and kinship testing with degraded DNA.International journal of legal medicine · 2013Article
- Development and validation of I-DNA1: a 15-Loci multiplex system for identity testing.International journal of legal medicine · 2011Article
- Haplotype-assisted characterization of germline mutations at short tandem repeat loci.International journal of legal medicine · 2010Article
- Meiosis study in a population sample from Afghanistan: allele frequencies and mutation rates of 16 STR loci.International journal of legal medicine · 2006Article
- Short amplicon STR multiplex for stain typing.International journal of legal medicine · 2006Article
- Low-volume amplification on chemically structured chips using the PowerPlex16 DNA amplification kit.International journal of legal medicine · 2006Article
- Characterisation of variant alleles in the STR systems D2S1338, D3S1358 and D19S433.International journal of legal medicine · 2005Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study the development of a 13-locus multiplex-PCR system fitting the updated demands for paternity testing in Germany is described. For this purpose an existing multiplex PCR system that allows the simultaneous amplification of eight different STR loci together with the sex-specific locus amelogenin ( genRESMPX-2, Serac, Germany) was extended. Whereas some of the primers were taken from the underlying multiplex system, suitable primer sequences were chosen for the STR loci D19S433, TPOX, TH01, D16S539, D5S818, D2S1338 and FGA. Primers of loci resulting in potentially overlapping fragment sizes were labelled with the fluorescent dyes 6-FAM, JOE and NED. Reaction conditions, such as annealing temperature, concentrations of primers and polymerase or buffer conditions were optimised to obtain a robust amplification and reproducible genotype analysis for various sample sources. Full DNA profiles from single source samples were reliably typed from template DNA amounts of as low as 120 pg, suggesting a potential use of this system also in forensic casework analysis. With a mean exclusion chance (MEC) of 99.9989% and a power of discrimination (P(D)) of about 1x10(14) (Caucasians), the new multiplex PCR system provides a significant and sensitive system for forensic DNA analysis. On the basis of these studies, a commercial kit system is now provided by Serac (Bad Homburg, Germany, genRESMPX-3).
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