ArticleJournal of virology2004
Effects of blocking individual maturation cleavages in murine leukemia virus gag.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 36 citations in OpenAlex.
- Reactivated endogenous retroviruses promote protein aggregate spreading.Nature communications · 2023Article
- Dominant Negative MA-CA Fusion Protein Is Incorporated into HIV-1 Cores and Inhibits Nuclear Entry of Viral Preintegration Complexes.Journal of virology · 2019Article
- Structure and architecture of immature and mature murine leukemia virus capsids.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Phosphorylation Requirement of Murine Leukemia Virus p12.Journal of virology · 2016Article
- p12 tethers the murine leukemia virus pre-integration complex to mitotic chromosomes.PLoS pathogens · 2012Article
- The gammaretroviral p12 protein has multiple domains that function during the early stages of replication.Retrovirology · 2012Article
- Clathrin facilitates the morphogenesis of retrovirus particles.PLoS pathogens · 2011Article
- On the role of the SP1 domain in HIV-1 particle assembly: a molecular switch?Journal of virology · 2011Article
- Ordered assembly of murine leukemia virus capsid protein on lipid nanotubes directs specific binding by the restriction factor, Fv1.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Murine leukemia viruses: objects and organisms.Advances in virology · 2011Article
- Protein transduction from retroviral Gag precursors.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Assembly properties of human immunodeficiency virus type 1 Gag-leucine zipper chimeras: implications for retrovirus assembly.Journal of virology · 2009Article
- Suboptimal inhibition of protease activity in human immunodeficiency virus type 1: effects on virion morphogenesis and RNA maturation.Virology · 2008Article
- Interactions of murine APOBEC3 and human APOBEC3G with murine leukemia viruses.Journal of virology · 2008Article
- Article
- Mutational analysis of the N-terminal domain of Moloney murine leukemia virus capsid protein.Journal of virology · 2007Article
- Overlapping roles of the Rous sarcoma virus Gag p10 domain in nuclear export and virion core morphology.Journal of virology · 2007Article
- Capsid is an important determinant for functional complementation of murine leukemia virus and spleen necrosis virus Gag proteins.Virology · 2007Article
- Effects of Gag mutation and processing on retroviral dimeric RNA maturation.Journal of virology · 2006Article
- Mutations affecting cleavage at the p10-capsid protease cleavage site block Rous sarcoma virus replication.Retrovirology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
A single protein, termed Gag, is responsible for retrovirus particle assembly. After the assembled virion is released from the cell, Gag is cleaved at several sites by the viral protease (PR). The cleavages catalyzed by PR bring about a wide variety of physical changes in the particle, collectively termed maturation, and convert the particle into an infectious virion. In murine leukemia virus (MLV) maturation, Gag is cleaved at three sites, resulting in formation of the matrix (MA), p12, capsid (CA), and nucleocapsid (NC) proteins. We introduced mutations into MLV that inhibited cleavage at individual sites in Gag. All mutants had lost the intensely staining ring characteristic of immature particles; thus, no single cleavage event is required for this feature of maturation. Mutant virions in which MA was not cleaved from p12 were still infectious, with a specific infectivity only approximately 10-fold below that of the wild type. Particles in which p12 and CA could not be separated from each other were noninfectious and lacked a well-delineated core despite the presence of dense material in their interiors. In both of these mutants, the dimeric viral RNA had undergone the stabilization normally associated with maturation, suggesting that this change may depend upon the separation of CA from NC. Alteration of the C-terminal end of CA blocked CA-NC cleavage but also reduced the efficiency of particle formation and, in some cases, severely disrupted the ability of Gag to assemble into regular structures. This observation highlights the critical role of this region of Gag in assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.