ArticleJournal of virology2004
Replication of poliovirus RNA with complete internal ribosome entry site deletions.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- UGA4 and YBR062C Regulate Oxidative Stress Tolerance Under Heavy Metal Toxicity.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Development and comprehensive evaluation of scarless circularization systems for circular RNA therapeutics.Molecular therapy. Nucleic acids · 2025Article
- Antivirotics based on defective interfering particles: emerging concepts and challenges.Frontiers in cellular and infection microbiology · 2025Review
- Engineering circular RNA for enhanced protein production.Nature biotechnology · 2023Article
- The enterovirus genome can be translated in an IRES-independent manner that requires the initiation factors eIF2A/eIF2D.PLoS biology · 2023Article
- Structure of the 5' Untranslated Region of Enteroviral Genomic RNA.Journal of virology · 2019Article
- Mutations in VP1 and 5'-UTR affect enterovirus 71 virulence.Scientific reports · 2018Article
- HnRNP A1 Alters the Structure of a Conserved Enterovirus IRES Domain to Stimulate Viral Translation.Journal of molecular biology · 2017Article
- Identification of a nucleotide in 5' untranslated region contributing to virus replication and virulence of Coxsackievirus A16.Scientific reports · 2016Article
- Mutational Disruption of cis-Acting Replication Element 2C in Coxsackievirus B3 Leads to 5'-Terminal Genomic Deletions.Journal of virology · 2015Article
- Evolution and Emergence of Enteroviruses through Intra- and Inter-species Recombination: Plasticity and Phenotypic Impact of Modular Genetic Exchanges in the 5' Untranslated Region.PLoS pathogens · 2015Article
- Major alteration in coxsackievirus B3 genomic RNA structure distinguishes a virulent strain from an avirulent strain.Nucleic acids research · 2014Article
- Role of RNA structure motifs in IRES-dependent translation initiation of the coxsackievirus B3: new insights for developing live-attenuated strains for vaccines and gene therapy.Molecular biotechnology · 2013Review
- Article
- Cis-active RNA elements (CREs) and picornavirus RNA replication.Virus research · 2009Review
- Poliovirus cis-acting replication element-dependent VPg Uridylylation lowers the Km of the initiating nucleoside triphosphate for viral RNA replication.Journal of virology · 2008Article
- 5' terminal deletions in the genome of a coxsackievirus B2 strain occurred naturally in human heart.Virology · 2008Article
- Poly(rC) binding proteins and the 5' cloverleaf of uncapped poliovirus mRNA function during de novo assembly of polysomes.Journal of virology · 2008Article
- Long-distance RNA-RNA interactions between terminal elements and the same subset of internal elements on the potato virus X genome mediate minus- and plus-strand RNA synthesis.RNA (New York, N.Y.) · 2007Article
- Structure of the 5' nontranslated region of the coxsackievirus b3 genome: Chemical modification and comparative sequence analysis.Journal of virology · 2007Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
cis-acting RNA sequences and structures in the 5' and 3' nontranslated regions of poliovirus RNA interact with host translation machinery and viral replication proteins to coordinately regulate the sequential translation and replication of poliovirus RNA. The poliovirus internal ribosome entry site (IRES) in the 5' nontranslated region (NTR) has been implicated as a cis-active RNA required for both viral mRNA translation and viral RNA replication. To evaluate the role of the IRES in poliovirus RNA replication, we exploited the advantages of cell-free translation-replication reactions and preinitiation RNA replication complexes. Genetic complementation with helper mRNAs allowed us to create preinitiation RNA replication complexes containing RNA templates with defined deletions in the viral open reading frame and the IRES. A series of deletions revealed that no RNA elements of either the viral open reading frame or the IRES were required in cis for negative-strand RNA synthesis. The IRES was dispensable for both negative- and positive-strand RNA syntheses. Intriguingly, although small viral RNAs lacking the IRES replicated efficiently, the replication of genome length viral RNAs was stimulated by the presence of the IRES. These results suggest that RNA replication is not directly dependent on a template RNA first functioning as an mRNA. These results further suggest that poliovirus RNA replication is not absolutely dependent on any protein-RNA interactions involving the IRES.
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