ArticleJournal of virology2004
Template sequence near the initiation nucleotide can modulate brome mosaic virus RNA accumulation in plant protoplasts.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Conserved motifs in a tombusvirus polymerase modulate genome replication, subgenomic transcription, and amplification of defective interfering RNAs.Journal of virology · 2015Article
- Common and unique features of viral RNA-dependent polymerases.Cellular and molecular life sciences : CMLS · 2014Review
- The plant host can affect the encapsidation of brome mosaic virus (BMV) RNA: BMV virions are surprisingly heterogeneous.Journal of molecular biology · 2014Article
- Mutations in the coat protein-binding cis-acting RNA motifs debilitate RNA recombination of Brome mosaic virus.Virus research · 2012Article
- Recombination of 5' subgenomic RNA3a with genomic RNA3 of Brome mosaic bromovirus in vitro and in vivo.Virology · 2011Article
- The 3'-terminal sequence of Bamboo mosaic virus minus-strand RNA interacts with RNA-dependent RNA polymerase and initiates plus-strand RNA synthesis.Molecular plant pathology · 2010Article
- Insights into the single-cell reproduction cycle of members of the family Bromoviridae: lessons from the use of protoplast systems.Journal of virology · 2008Review
- cis- and trans-acting functions of brome mosaic virus protein 1a in genomic RNA1 replication.Journal of virology · 2008Article
- Replication-independent long-distance trafficking by viral RNAs in Nicotiana benthamiana.The Plant cell · 2007Article
- A multicomponent RNA-based control system regulates subgenomic mRNA transcription in a tombusvirus.Journal of virology · 2007Article
- RNA-binding proteins that inhibit RNA virus infection.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Long-distance RNA-RNA interactions between terminal elements and the same subset of internal elements on the potato virus X genome mediate minus- and plus-strand RNA synthesis.RNA (New York, N.Y.) · 2007Article
- Selective repression of translation by the brome mosaic virus 1a RNA replication protein.Journal of virology · 2007Article
- In vivo packaging of brome mosaic virus RNA3, but not RNAs 1 and 2, is dependent on a cis-acting 3' tRNA-like structure.Journal of virology · 2007Article
- RNA editing complex interactions with a site for full-round U deletion in Trypanosoma brucei.RNA (New York, N.Y.) · 2006Article
- Interaction between Brome mosaic virus proteins and RNAs: effects on RNA replication, protein expression, and RNA stability.Journal of virology · 2005Article
- Mutual interference between genomic RNA replication and subgenomic mRNA transcription in brome mosaic virus.Journal of virology · 2005Article
- Repair of the tRNA-like CCA sequence in a multipartite positive-strand RNA virus.Journal of virology · 2005Article
- Replicase-binding sites on plus- and minus-strand brome mosaic virus RNAs and their roles in RNA replication in plant cells.Journal of virology · 2004Article
- Dissecting the requirement for subgenomic promoter sequences by RNA recombination of brome mosaic virus in vivo: evidence for functional separation of transcription and recombination.Journal of virology · 2004Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Bromoviral templates for plus-strand RNA synthesis are rich in A or U nucleotides in comparison to templates for minus-strand RNA synthesis. Previous studies demonstrated that plus-strand RNA synthesis by the brome mosaic virus (BMV) RNA replicase is more efficient if the template contains an A/U-rich template sequence near the initiation site (K. Sivakumaran and C. C. Kao, J. Virol. 73:6415-6423, 1999). These observations led us to examine the effects of nucleotide changes near the template's initiation site on the accumulation of BMV RNA3 genomic minus-strand, genomic plus-strand, and subgenomic RNAs in barley protoplasts transfected with wild-type and mutant BMV transcripts. Mutations in the template for minus-strand synthesis had only modest effects on BMV replication in barley protoplasts. Mutants with changes to the +3, +5, and +7 template nucleotides accumulated minus-strand RNA at levels similar to the the wild-type level. However, mutations at positions adjacent to the initiation cytidylate in the templates for genomic and subgenomic plus-strand RNA synthesis significantly decreased RNA accumulation. For example, changes at the third template nucleotide for plus-strand RNA3 synthesis resulted in RNA accumulation at between 18 and 24% of the wild-type level, and mutations in the third template nucleotide for subgenomic RNA4 resulted in accumulations at between 7 and 14% of the wild-type level. The effects of the mutations generally decreased as the mutations occurred further from the initiation nucleotide. These findings demonstrate that there are different requirements of the template sequence near the initiation nucleotide for BMV RNA accumulation in plant cells.
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