ArticleMolecular biology of the cell2004
Adenylyl cyclase G is activated by an intramolecular osmosensor.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Article
- Adenylate cyclase A amplification and functional diversification during Polyspondylium pallidum development.EvoDevo · 2022Article
- Article
- NFAT5 genes are part of the osmotic regulatory system in Atlantic salmon (Salmo salar).Marine genomics · 2017Article
- A conserved signalling pathway for amoebozoan encystation that was co-opted for multicellular development.Scientific reports · 2015Article
- Osmosensing and osmoregulation in unicellular eukaryotes.World journal of microbiology & biotechnology · 2015Review
- The ABC transporter, AbcB3, mediates cAMP export in D. discoideum development.Developmental biology · 2015Article
- The Amoebozoa.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Identification and characterization of peptide: N-glycanase from Dictyostelium discoideum.BMC biochemistry · 2012Article
- Evolution of developmental cyclic adenosine monophosphate signaling in the Dictyostelia from an amoebozoan stress response.Development, growth & differentiation · 2011Review
- Deficiency of huntingtin has pleiotropic effects in the social amoeba Dictyostelium discoideum.PLoS genetics · 2011Article
- Functional dissection of adenylate cyclase R, an inducer of spore encapsulation.The Journal of biological chemistry · 2010Article
- From drought sensing to developmental control: evolution of cyclic AMP signaling in social amoebas.Molecular biology and evolution · 2008Article
- cAMP production by adenylyl cyclase G induces prespore differentiation in Dictyostelium slugs.Development (Cambridge, England) · 2007Article
- Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP.The Biochemical journal · 2007Article
- Pharmacological profiling of the Dictyostelium adenylate cyclases ACA, ACB and ACG.The Biochemical journal · 2007Article
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2 authors.
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Abstract
Adenylyl cyclase G (ACG) is activated by high osmolality and mediates inhibition of spore germination by this stress factor. The catalytic domains of all eukaryote cyclases are active as dimers and dimerization often mediates activation. To investigate the role of dimerization in ACG activation, we coexpressed ACG with an ACG construct that lacked the catalytic domain (ACGDeltacat) and was driven by a UV-inducible promoter. After UV induction of ACGDeltacat, cAMP production by ACG was strongly inhibited, but osmostimulation was not reduced. Size fractionation of native ACG showed that dimers were formed between ACG molecules and between ACG and ACGDeltacat. However, high osmolality did not alter the dimer/monomer ratio. This indicates that ACG activity requires dimerization via a region outside the catalytic domain but that dimer formation does not mediate activation by high osmolality. To establish whether ACG required auxiliary sensors for osmostimulation, we expressed ACG cDNA in a yeast adenylyl cyclase null mutant. In yeast, cAMP production by ACG was similarly activated by high osmolality as in Dictyostelium. This strongly suggests that the ACG osmosensor is intramolecular, which would define ACG as the first characterized primary osmosensor in eukaryotes.
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