ArticleMolecular biology of the cell2004
The differential roles of budding yeast Tem1p, Cdc15p, and Bub2p protein dynamics in mitotic exit.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 92 citations in OpenAlex.
- Proteome-wide forced interactions reveal a functional map of cell-cycle phospho-regulation inNucleus (Austin, Tex.) · 2024Article
- A noncanonical GTPase signaling mechanism controls exit from mitosis in budding yeast.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A noncanonical GTPase signaling mechanism controls exit from mitosis in budding yeast.bioRxiv : the preprint server for biology · 2024Article
- The signalling lipid PI3,5POpen biology · 2023Article
- SWR1 chromatin remodeling complex prevents mitotic slippage during spindle position checkpoint arrest.Molecular biology of the cell · 2023Article
- Phosphosites of the yeast centrosome component Spc110 contribute to cell cycle progression and mitotic exit.Biology open · 2022Article
- The budding yeast GSK-3 homologue Mck1 is an essential component of the spindle position checkpoint.Open biology · 2022Article
- Article
- Article
- Spindle pole bodies function as signal amplifiers in the Mitotic Exit Network.Molecular biology of the cell · 2020Article
- Review
- Budding YeastG3 (Bethesda, Md.) · 2018Article
- Temporal and compartment-specific signals coordinate mitotic exit with spindle position.Nature communications · 2017Article
- Assembly, molecular organization, and membrane-binding properties of development-specific septins.The Journal of cell biology · 2016Article
- Coupling spindle position with mitotic exit in budding yeast: The multifaceted role of the small GTPase Tem1.Small GTPases · 2015Review
- Asymmetry of the budding yeast Tem1 GTPase at spindle poles is required for spindle positioning but not for mitotic exit.PLoS genetics · 2015Article
- Dual Regulation of the mitotic exit network (MEN) by PP2A-Cdc55 phosphatase.PLoS genetics · 2013Article
- Mitotic exit and separation of mother and daughter cells.Genetics · 2012Review
- Control of the mitotic exit network during meiosis.Molecular biology of the cell · 2012Article
- Astral microtubule asymmetry provides directional cues for spindle positioning in budding yeast.Experimental cell research · 2012Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
In the budding yeast Saccharomyces cerevisiae the mitotic spindle must be positioned along the mother-bud axis to activate the mitotic exit network (MEN) in anaphase. To examine MEN proteins during mitotic exit, we imaged the MEN activators Tem1p and Cdc15p and the MEN regulator Bub2p in vivo. Quantitative live cell fluorescence microscopy demonstrated the spindle pole body that segregated into the daughter cell (dSPB) signaled mitotic exit upon penetration into the bud. Activation of mitotic exit was associated with an increased abundance of Tem1p-GFP and the localization of Cdc15p-GFP on the dSPB. In contrast, Bub2p-GFP fluorescence intensity decreased in mid-to-late anaphase on the dSPB. Therefore, MEN protein localization fluctuates to switch from Bub2p inhibition of mitotic exit to Cdc15p activation of mitotic exit. The mechanism that elevates Tem1p-GFP abundance in anaphase is specific to dSPB penetration into the bud and Dhc1p and Lte1p promote Tem1p-GFP localization. Finally, fluorescence recovery after photobleaching (FRAP) measurements revealed Tem1p-GFP is dynamic at the dSPB in late anaphase. These data suggest spindle pole penetration into the bud activates mitotic exit, resulting in Tem1p and Cdc15p persistence at the dSPB to initiate the MEN signal cascade.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.