ArticleMolecular biology of the cell2004
The yeast elongator histone acetylase requires Sit4-dependent dephosphorylation for toxin-target capacity.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 61 citations in OpenAlex.
- Autophosphorylation of conserved yeast and human casein kinase 1 isozymes regulates Elongator-dependent tRNA modifications.Nucleic acids research · 2025Article
- tRNA binding to Kti12 is crucial for wobble uridine modification by Elongator.Nucleic acids research · 2025Article
- Cryo-EM structure of the fully assembled Elongator complex.Nucleic acids research · 2023Article
- Anticodon Wobble Uridine Modification by Elongator at the Crossroad of Cell Signaling, Differentiation, and Diseases.Epigenomes · 2020Review
- Kti12, a PSTK-like tRNA dependent ATPase essential for tRNA modification by Elongator.Nucleic acids research · 2019Article
- Roles of Elongator Dependent tRNA Modification Pathways in Neurodegeneration and Cancer.Genes · 2018Review
- Protein Phosphatase Sit4 Affects Lipid Droplet Synthesis and Soraphen A Resistance Independent of Its Role in Regulating Elongator Dependent tRNA Modification.Biomolecules · 2018Article
- Use of a Yeast tRNase Killer Toxin to Diagnose Kti12 Motifs Required for tRNA Modification by Elongator.Toxins · 2017Article
- Independent suppression of ribosomal +1 frameshifts by different tRNA anticodon loop modifications.RNA biology · 2017Article
- Wobble uridine modifications-a reason to live, a reason to die?!RNA biology · 2017Review
- Sulfur transfer and activation by ubiquitin-like modifier system Uba4•Urm1 link protein urmylation and tRNA thiolation in yeast.Microbial cell (Graz, Austria) · 2016Article
- Yeast Elongator protein Elp1p does not undergo proteolytic processing in exponentially growing cells.MicrobiologyOpen · 2015Article
- Elongator-dependent modification of cytoplasmic tRNALysUUU is required for mitochondrial function under stress conditions.Nucleic acids research · 2015Article
- Phosphorylation of Elp1 by Hrr25 is required for elongator-dependent tRNA modification in yeast.PLoS genetics · 2015Article
- The emerging role of complex modifications of tRNAMicrobial cell (Graz, Austria) · 2015Article
- Loss of anticodon wobble uridine modifications affects tRNA(Lys) function and protein levels in Saccharomyces cerevisiae.PloS one · 2015Article
- Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling.Microbial cell (Graz, Austria) · 2014Article
- Elongator, a conserved complex required for wobble uridine modifications in eukaryotes.RNA biology · 2014Review
- Transfer RNA post-transcriptional processing, turnover, and subcellular dynamics in the yeast Saccharomyces cerevisiae.Genetics · 2013Review
- A novel Sit4 phosphatase complex is involved in the response to ceramide stress in yeast.Oxidative medicine and cellular longevity · 2013Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kluyveromyces lactis zymocin, a heterotrimeric toxin complex, imposes a G1 cell cycle block on Saccharomyces cerevisiae that requires the toxin-target (TOT) function of holo-Elongator, a six-subunit histone acetylase. Here, we demonstrate that Elongator is a phospho-complex. Phosphorylation of its largest subunit Tot1 (Elp1) is supported by Kti11, an Elongator-interactor essential for zymocin action. Tot1 dephosphorylation depends on the Sit4 phosphatase and its associators Sap185 and Sap190. Zymocin-resistant cells lacking or overproducing Elongator-associator Tot4 (Kti12), respectively, abolish or intensify Tot1 phosphorylation. Excess Sit4.Sap190 antagonizes the latter scenario to reinstate zymocin sensitivity in multicopy TOT4 cells, suggesting physical competition between Sit4 and Tot4. Consistently, Sit4 and Tot4 mutually oppose Tot1 de-/phosphorylation, which is dispensable for integrity of holo-Elongator but crucial for the TOT-dependent G1 block by zymocin. Moreover, Sit4, Tot4, and Tot1 cofractionate, Sit4 is nucleocytoplasmically localized, and sit4Delta-nuclei retain Tot4. Together with the findings that sit4Delta and totDelta cells phenocopy protection against zymocin and the ceramide-induced G1 block, Sit4 is functionally linked to Elongator in cell cycle events targetable by antizymotics.
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