ArticleNucleic acids research2004
The guanine-rich fragile X chromosome repeats are reluctant to form tetraplexes.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Biophysics in the Czech Republic: 70th anniversary of the institute of biophysics.European biophysics journal : EBJ · 2025Review
- Structural and Dynamical Properties of Nucleic Acid Hairpins Implicated in Trinucleotide Repeat Expansion Diseases.Biomolecules · 2024Review
- Structural effects of inosine substitution in telomeric DNA quadruplex.Frontiers in chemistry · 2024Article
- G-Quadruplexes in Repeat Expansion Disorders.International journal of molecular sciences · 2023Review
- Roles of G4-DNA and G4-RNA in Class Switch Recombination and Additional Regulations in B-Lymphocytes.Molecules (Basel, Switzerland) · 2023Review
- The mechanism of replication stalling and recovery within repetitive DNA.Nature communications · 2022Article
- Novel eGZ-motif formed by regularly extruded guanine bases in a left-handed Z-DNA helix as a major motif behind CGG trinucleotide repeats.Nucleic acids research · 2022Article
- Secondary structural choice of DNA and RNA associated with CGG/CCG trinucleotide repeat expansion rationalizes the RNA misprocessing in FXTAS.Scientific reports · 2021Article
- Structures and stability of simple DNA repeats from bacteria.The Biochemical journal · 2020Review
- A guide to computational methods for G-quadruplex prediction.Nucleic acids research · 2020Review
- CoInternational journal of molecular sciences · 2018Article
- Exploring possible DNA structures in real-time polymerase kinetics using Pacific Biosciences sequencer data.BMC bioinformatics · 2015Article
- An RNA hairpin to G-quadruplex conformational transition.Journal of the American Chemical Society · 2012Article
- G-quadruplex nucleic acids and human disease.The FEBS journal · 2010Review
- AGG interruptions in (CGG)(n) DNA repeat tracts modulate the structure and thermodynamics of non-B conformations in vitro.Biochemistry · 2010Article
- Genome-wide analyses of recombination prone regions predict role of DNA structural motif in recombination.PloS one · 2009Article
- Putative DNA quadruplex formation within the human c-kit oncogene.Journal of the American Chemical Society · 2005Article
- Length-dependent energetics of (CTG)n and (CAG)n trinucleotide repeats.Nucleic acids research · 2005Article
- Prevalence of quadruplexes in the human genome.Nucleic acids research · 2005Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Using circular dichroism spectroscopy, UV absorption spectroscopy and polyacrylamide gel electrophoresis, we studied conformational properties of guanine-rich DNA strands of the fragile X chromosome repeats d(GGC)n, d(GCG)n and d(CGG)n, with n = 2, 4, 8 and 16. These strands are generally considered in the literature to form guanine tetraplexes responsible for the repeat expansion. However, we show in this paper that the repeats are reluctant to form tetraplexes. At physiological concentrations of either Na+ or K+ ions, the hexamers and dodecamers associate to form homoduplexes and the longer repeats generate homoduplexes and hairpins. The tetraplexes are rarely observed being relatively most stable with d(GGC)n and least stable with d(GCG)n. The tetraplexes are exclusively formed in the presence of K+ ions, at salt concentrations higher than physiological, more easily at higher than physiological temperatures, and they arise with extremely long kinetics (even days). Moreover, the capability to form tetraplexes sharply diminishes with the oligonucleotide length. These facts make the concept of the tetraplex appearance in this motif in vivo very improbable. Rather, a hairpin of the fragile X repeats, whose stability increases with the repeat length, is the probable structure responsible for the repeat expansion in genomes.
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