ArticleNucleic acids research2004
Characterization of cytosine methylated regions and 5-cytosine DNA methyltransferase (Ehmeth) in the protozoan parasite Entamoeba histolytica.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 122 citations in OpenAlex.
- Gene regulation inCurrent research in parasitology & vector-borne diseases · 2025Article
- Joint Analysis of Genome-wide DNA Methylation and Transcription Sequencing Identifies the Role of BAX Gene in Heat Stress-Induced-Sertoli Cells Apoptosis.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- Characterization of the dual role of Plasmodium falciparum DNA methyltransferase in regulating transcription and translation.Nucleic acids research · 2023Article
- Advances in Protozoan Epigenetic Targets and Their Inhibitors for the Development of New Potential Drugs.Pharmaceuticals (Basel, Switzerland) · 2023Review
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- Are Metabolites From the Gut Microbiota Capable of Regulating Epigenetic Mechanisms in the Human ParasiteFrontiers in cell and developmental biology · 2022Review
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- Adenine DNA methylation, 3D genome organization, and gene expression in the parasiteProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Transcriptome Analysis ofFrontiers in plant science · 2020Article
- Methylation-reprogrammed Wnt/β-catenin signalling mediated prenatal hypoxia-induced brain injury in foetal and offspring rats.Journal of cellular and molecular medicine · 2018Article
- The anti-fecundity effect of 5-azacytidine (5-AzaC) on Schistosoma mansoni is linked to dis-regulated transcription, translation and stem cell activities.International journal for parasitology. Drugs and drug resistance · 2018Article
- Evolution of DNMT2 in drosophilids: Evidence for positive and purifying selection and insights into new protein (pathways) interactions.Genetics and molecular biology · 2018Article
- Widespread 5-methylcytosine in the genomes of avian Coccidia and other apicomplexan parasites detected by an ELISA-based method.Parasitology research · 2017Article
- The Biomphalaria glabrata DNA methylation machinery displays spatial tissue expression, is differentially active in distinct snail populations and is modulated by interactions with Schistosoma mansoni.PLoS neglected tropical diseases · 2017Article
- DNA Methylation of Gene Expression inThe Korean journal of parasitology · 2017Article
- Article
- Characterization of Cytosine Methylation and the DNA Methyltransferases ofInternational journal of biological sciences · 2017Article
- Identification of Protein Arginine Methyltransferase 5 as a Regulator for Encystation of Acanthamoeba.The Korean journal of parasitology · 2016Article
- Identification of repressive and active epigenetic marks and nuclear bodies in Entamoeba histolytica.Parasites & vectors · 2016Article
- Entamoeba histolytica: protein arginine transferase 1a methylates arginine residues and potentially modify the H4 histone.Parasites & vectors · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The DNA methylation status of the protozoan parasite Entamoeba histolytica was heretofore unknown. In the present study, we developed a new technique, based on the affinity of methylated DNA to 5-methylcytosine antibodies, to identify methylated DNA in this parasite. Ribosomal DNA and ribosomal DNA circles were isolated by this method and we confirmed the validity of our approach by sodium bisulfite sequencing. We also report the identification and the characterization of a gene, Ehmeth, encoding a DNA methyltransferase strongly homologous to the human DNA methyltransferase 2 (Dnmt2). Immunofluorescence microscopy using an antibody raised against a recombinant Ehmeth showed that Ehmeth is concentrated in the nuclei of trophozoites. The recombinant Ehmeth has a weak but significant methyltransferase activity when E.histolytica genomic DNA is used as substrate. 5-Azacytidine (5-AzaC), an inhibitor of DNA methyltransferase, was used to study in vivo the role of DNA methylation in E.histolytica. Genomic DNA of trophozoites grown with 5-AzaC (23 microM) was undermethylated and the ability of 5-AzaC-treated trophozoites to kill mammalian cells or to cause liver abscess in hamsters was strongly impaired.
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