ArticleJournal of clinical microbiology2004
Development of a single-tube, cell lysis-based, genus-specific PCR method for rapid identification of mycobacteria: optimization of cell lysis, PCR primers and conditions, and restriction pattern analysis.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Microbial Community Establishment, Succession, and Temporal Dynamics in an Industrial Semi-Synthetic Metalworking Fluid Operation: A 50-Week Real-Time Tracking.Microorganisms · 2024Article
- Screening, molecular identification, population diversity, and antibiotic susceptibility pattern of Actinomycetes species isolated from meat and meat products of slaughterhouses, restaurants, and meat stores of a developing country, Iran.Frontiers in microbiology · 2023Article
- Species diversity and molecular analysis of opportunistic Mycobacterium, Nocardia and Rhodococcus isolated from the hospital environment in a developing country, a potential resources for nosocomial infection.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021Article
- A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast.eLife · 2020Article
- Species Diversity, Molecular Characterization, and Antimicrobial Susceptibility of Opportunistic Actinomycetes Isolated from Immunocompromised and Healthy Patients of Markazi Province of Iran.Infection and drug resistance · 2020Article
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- Article
- Recent Developments in Single-Cell RNA-Seq of Microorganisms.Biophysical journal · 2018Review
- Complete Genome Sequences of the Probiotic Lactic Acid BacteriaGenome announcements · 2018Article
- Screening, isolation and molecular identification of biodegrading mycobacteria from Iranian ecosystems and analysis of their biodegradation activity.AMB Express · 2017Article
- Tools for Genomic and Transcriptomic Analysis of Microbes at Single-Cell Level.Frontiers in microbiology · 2017Review
- Article
- Molecular Identification of Clinical Isolates of Mycobacterium fortuitum by Random Amplified Polymorphic DNA (RAPD) Polymerase Chain Reaction and ERIC PCR.Journal of clinical and diagnostic research : JCDR · 2015Article
- Molecular typing of Iranian mycobacteria isolates by polymerase chain reaction-restriction fragment length polymorphism analysis of 360-bp rpoB gene.Advanced biomedical research · 2015Article
- Isolation and molecular identification of biodegrading Mycobacteria from water supplies of Iranian hospitals.Iranian journal of microbiology · 2014Article
- Identification of Nontuberculous Mycobacteria Species Isolated from Water Samples Using Phenotypic and Molecular Methods and Determination of their Antibiotic Resistance Patterns by E- Test Method, in Isfahan, Iran.Iranian journal of basic medical sciences · 2012Article
- Molecular Identification of Rare Clinical Mycobacteria by Application of 16S-23S Spacer Region Sequencing.Iranian journal of basic medical sciences · 2012Article
- Chronic pulmonary disease due to Mycobacterium monacense infection: the first case from Iran.Annals of laboratory medicine · 2012Article
- Mycobacterium novocastrense-associated pulmonary and wound infections.Emerging infectious diseases · 2011Article
- Rapid identification of the Mycobacterium tuberculosis complex by combining the ESAT-6/CFP-10 immunochromatographic assay and smear morphology.Journal of clinical microbiology · 2011Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
A single-tube PCR method was developed for efficient identification of nontuberculous mycobacteria (NTM) and their environmental isolates in about 3 h without conventional DNA isolation. The following three steps were optimized or developed: (i). a simple, 6-min direct cell lysis protocol as a PCR prestep for generation of DNA-template, (ii). an improved Mycobacterium-specific PCR amplification protocol with a broader species specificity using newly designed primers targeting a 228-bp region of the 65-kDa heat shock protein (hsp) gene and optimal PCR amplification conditions, and (iii). a genus-specific restriction analysis of the PCR product for conclusive identification of the unknown NTM isolates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.