ArticleJournal of clinical microbiology2004
Impact of DNA polymerases and their buffer systems on quantitative real-time PCR.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- USTAT: Unified Sequential Template Amplification and Transcription-a fully synthetic mRNA manufacturing platform.NPJ vaccines · 2026Article
- Article
- PCR-Based Equine Gene Doping Test for the Australian Horseracing Industry.International journal of molecular sciences · 2024Article
- IMPLANT: a new technique for transgene copy number estimation in plants using a single end-point PCR reaction.Plant methods · 2022Article
- Wastewater-based epidemiology in countries with poor wastewater treatment - Epidemiological indicator function of SARS-CoV-2 RNA in surface waters.The Science of the total environment · 2022Article
- Identification of Novel Endogenous Controls for qPCR Normalization in SK-BR-3 Breast Cancer Cell Line.Genes · 2021Article
- Comparison of Seven Commercial TaqMan Master Mixes and Two Real-Time PCR Platforms Regarding the Rapid Detection of Porcine DNA.Food science of animal resources · 2021Review
- PCR inhibition in qPCR, dPCR and MPS-mechanisms and solutions.Analytical and bioanalytical chemistry · 2020Review
- Essential role of polymerases for assay performance - Impact of polymerase replacement in a well-established assay.Biomolecular detection and quantification · 2018Article
- Preliminary analysis of New Zealand scampi (PeerJ · 2018Article
- Identifying suitable reference genes for gene expression analysis in developing skeletal muscle in pigs.PeerJ · 2016Article
- Molecular breeding of polymerases for resistance to environmental inhibitors.Nucleic acids research · 2011Article
- Comparative analysis of four methods to extract DNA from paraffin-embedded tissues: effect on downstream molecular applications.BMC research notes · 2010Article
- Production of random DNA oligomers for scalable DNA computing.Biotechnology journal · 2009Article
- Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon.BMC research notes · 2008Article
- Enumeration of salmonella bacteria in food and feed samples by real-time PCR for quantitative microbial risk assessment.Applied and environmental microbiology · 2008Review
- Direct quantitation and detection of salmonellae in biological samples without enrichment, using two-step filtration and real-time PCR.Applied and environmental microbiology · 2006Article
- Rapid detection and sequence-specific differentiation of extended-spectrum beta-lactamase GES-2 from Pseudomonas aeruginosa by use of a real-time PCR assay.Antimicrobial agents and chemotherapy · 2004Article
- Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction.Journal of biomolecular techniques : JBT · 2004Review
- Rapid quantification of Yersinia enterocolitica in pork samples by a novel sample preparation method, flotation, prior to real-time PCR.Journal of clinical microbiology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.