ArticleJournal of clinical microbiology2004
Novel mass spectrometry-based tool for genotypic identification of mycobacteria.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 83 citations in OpenAlex.
- "Omic" Approaches to Bacteria and Antibiotic Resistance Identification.International journal of molecular sciences · 2022Review
- Comparison of Two Commercial Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Systems for Identification of Nontuberculous Mycobacteria.American journal of clinical pathology · 2019Article
- Ratiometric Mass Spectrometry for Cell Identification and Quantitation Using Intracellular "Dual-Biomarkers".Scientific reports · 2017Article
- Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria.Clinical microbiology reviews · 2016Review
- MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis.Frontiers in microbiology · 2015Review
- MALDI-TOF mass spectrometry proteomic based identification of clinical bacterial isolates.The Indian journal of medical research · 2014Article
- Rapid inactivation of Mycobacterium and nocardia species before identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry.Journal of clinical microbiology · 2014Article
- Performance of mass spectrometric identification of bacteria and yeasts routinely isolated in a clinical microbiology laboratory using MALDI-TOF MS.Journal of thoracic disease · 2014Article
- Review
- Application of mass spectrometry to molecular diagnostics of viral infections.Expert review of molecular diagnostics · 2013Review
- [Mass spectrometry in the clinical microbiology laboratory].Enfermedades infecciosas y microbiologia clinica · 2012Review
- Rapid identification of mycobacterial whole cells in solid and liquid culture media by matrix-assisted laser desorption ionization-time of flight mass spectrometry.Journal of clinical microbiology · 2010Article
- Identification of pathogens by mass spectrometry.Clinical chemistry · 2010Review
- High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories.Journal of clinical microbiology · 2010Article
- Identification of RNA molecules by specific enzyme digestion and mass spectrometry: software for and implementation of RNA mass mapping.Nucleic acids research · 2009Article
- [Microbiological diagnosis: Etiologic driven and syndrome driven].Antibiotiques (Paris, France : 1999) · 2009Article
- Occurrence and clinical relevance of Mycobacterium chimaera sp. nov., Germany.Emerging infectious diseases · 2008Article
- Abstracts of the 18th European Congress of Clinical Microbiology and Infectious Diseases. Barcelona, Spain. April 19-22, 2008.Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases · 2008Article
- Toward universal flavivirus identification by mass cataloging.The Journal of molecular diagnostics : JMD · 2008Article
- Automated comparative sequence analysis by base-specific cleavage and mass spectrometry for nucleic acid-based microbial typing.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) after base-specific cleavage of PCR amplified and in vitro-transcribed 16S rRNA gene (rDNA) was used for the identification of mycobacteria. Full-length 16S rDNA reference sequences of 12 type strains of Mycobacterium spp. frequently isolated from clinical specimens were determined by PCR, cloning, and sequencing. For MALDI-TOF MS-based comparative sequence analysis, mycobacterial 16S rDNA signature sequences ( approximately 500 bp) of the 12 type strains and 24 clinical isolates were PCR amplified using RNA promoter-tagged forward primers. T7 RNA polymerase-mediated transcription of forward strands in the presence of 5-methyl ribo-CTP maximized mass differences of fragments generated by base-specific cleavage. In vitro transcripts were subsequently treated with RNase T1, resulting in G-specific cleavage. Sample analysis by MALDI-TOF MS showed a specific mass signal pattern for each of the 12 type strains, allowing unambiguous identification. All 24 clinical isolates were identified unequivocally by comparing their detected mass signal pattern to the reference sequence-derived in silico pattern of the type strains and to the in silico mass patterns of published 16S rDNA sequences. A 16S rDNA microheterogeneity of the Mycobacterium xenopi type strain (DSM 43995) was detected by MALDI-TOF MS and later confirmed by Sanger dideoxy sequencing. In conclusion, analysis of 16S rDNA amplicons by MS after base-specific cleavage of RNA transcripts allowed fast and reliable identification of the Mycobacterium tuberculosis complex and ubiquitous mycobacteria (mycobacteria other than tuberculosis). The technology delivers an open platform for high-throughput microbial identification on the basis of any specific genotypic marker region.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.