ArticleJournal of clinical microbiology2004
Development of a real-time reverse-transcription PCR for detection of newcastle disease virus RNA in clinical samples.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 220 papers.
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Who cites it
220 citing papers in PubMed, 517 citations in OpenAlex.
- Detection of highly pathogenic avian influenza A(H5N1) virus, clade 2.3.4.4b, genotype B3.13 in placentas from aborted bovine fetuses.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2026Article
- First report of molecular genotyping, pathotyping, and histopathological characterization of lentogenic genotype II Newcastle disease virus circulating in young ostrich flocks in Egypt.Veterinary world · 2026Article
- Development and Analytical Validation of a Multiplex Real-Time RT-PCR Assay for Simultaneous Detection of Avian Influenza A Virus and Newcastle Disease Virus.Pathogens (Basel, Switzerland) · 2026Article
- Full genome sequencing, evolutionary dynamics, and pathogenicity evaluation of chicken infectious anemia virus with emphasis on Upper Egypt reveals genetic variability linked to vaccinal strains.Virology journal · 2026Article
- Long term circulation of avian paramyxovirus 4 in Australia revealed by historical and contemporary genomes from wild and domestic birds.Virology journal · 2026Article
- High-sensitivity multiplex real-time RT-PCR for universal detection and virulence typing of Newcastle disease virus in poultry field samples.Poultry science · 2026Article
- A genetically-distinct Pigeon Paramyxovirus-I isolate fails to cross-react with neutralizing antibodies raised by LaSota vaccine in chicken.Virus genes · 2026Article
- Biosecurity assessment and seroprevalence of relevant poultry diseases in Saint Kitts commercial poultry premises.Tropical animal health and production · 2026Article
- Prevalence, molecular characterization and phylogenetic analysis of Newcastle disease virus isolated from domestic poultry during 2019-2024 in Egypt.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Article
- Global evolution dynamics of genotype VI NDVs and dissection of the biological properties of strains from the prevalent sub-genotypes.Journal of virology · 2026Article
- Pathogenicity and Genotyping of Fowl Adenovirus-D Serotype 2/11 Circulating in Commercial Broilers in Egypt.Viruses · 2026Article
- Histopathological diagnosis and genetic diversity of avian encephalomyelitis virus circulating in broiler farms in Egypt.Open veterinary journal · 2026Article
- Isolation and Molecular Detection of Newcastle Disease Virus From Outbreak Cases in Selected Areas of Northern and Western Oromia, Ethiopia.Veterinary medicine and science · 2026Article
- Highly Virulent Newcastle Disease Virus in Eurasian Collared Doves in the North of Portugal.Animals : an open access journal from MDPI · 2025Article
- Genomic and evolutionary characterization of newly emerged highly pathogenic avian influenza H5N1 clade (2023-2025).Veterinary world · 2025Article
- Molecular detection of paramyxovirus and coronavirus in fecal samples of shorebirds from Southern Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Biosurveillance of avian orthoavulavirus 1 in multiple avian species in Pakistan from 2011 to 2021.Open veterinary journal · 2025Article
- Molecular characterization and genotyping of Newcastle disease viruses using whole-genome sequencing from poultry farms in parts of Amhara Regions, and central Ethiopia.Virology journal · 2025Article
- Draft genome sequences of four Newcastle disease virus isolates from commercial poultry farms in Ethiopia.Microbiology resource announcements · 2025Article
160 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A real-time reverse-transcription PCR (RRT-PCR) was developed to detect avian paramyxovirus 1 (APMV-1) RNA, also referred to as Newcastle disease virus (NDV), in clinical samples from birds. The assay uses a single-tube protocol with fluorogenic hydrolysis probes. Oligonucleotide primers and probes were designed to detect sequences from a conserved region of the matrix protein (M) gene that recognized a diverse set (n = 44) of APMV-1 isolates. A second primer-probe set was targeted to sequences in the fusion protein (F) gene that code for the cleavage site and detect potentially virulent NDV isolates. A third set, also directed against the M gene, was specific for the North American (N.A.) pre-1960 genotype that includes the common vaccine strains used in commercial poultry in the United States. The APMV-1 M gene, N.A. pre-1960 M gene, and F gene probe sets were capable of detecting approximately 10(3), 10(2), and 10(4) genome copies, respectively, with in vitro-transcribed RNA. Both M gene assays could detect approximately 10(1) 50% egg infective doses (EID(50)), and the F gene assay could detect approximately 10(3) EID(50). The RRT-PCR test was used to examine clinical samples from chickens experimentally infected with the NDV strain responsible for a recent epizootic in the southwestern United States. Overall, a positive correlation was obtained between the RRT-PCR results and virus isolation for NDV from clinical samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.