ArticleJournal of clinical microbiology2004
Evaluation of a TaqMan PCR assay to detect rabies virus RNA: influence of sequence variation and application to quantification of viral loads.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- A step forward in molecular diagnostics of lyssaviruses--results of a ring trial among European laboratories.PloS one · 2013Trial
- Prevalence and molecular epidemiology of rotavirus gastroenteritis among children in Nairobi's urban informal settlements following introduction of the Rotavac® vaccine.Tropical medicine and health · 2026Article
- The Challenge of Lyssavirus Infections in Domestic and Other Animals: A Mix of Virological Confusion, Consternation, Chagrin, and Curiosity.Pathogens (Basel, Switzerland) · 2025Review
- Recent updates on laboratory diagnosis of rabies.The Indian journal of medical research · 2024Review
- Detection of rabies virusFrontiers in veterinary science · 2022Article
- Multiple detection and spread of novel strains of the SARS-CoV-2 B.1.177 (B.1.177.75) lineage that test negative by a commercially available nucleocapsid gene real-time RT-PCR.Emerging microbes & infections · 2021Article
- Mutations in Animal SARS-CoV-2 Induce Mismatches with the Diagnostic PCR Assays.Pathogens (Basel, Switzerland) · 2021Article
- Article
- Presence of mismatches between diagnostic PCR assays and coronavirus SARS-CoV-2 genome.Royal Society open science · 2020Article
- Development of a quantitative real-time RT-PCR assay for detecting Taiwan ferret badger rabies virus in ear tissue of ferret badgers and mice.The Journal of veterinary medical science · 2018Article
- Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2017Article
- Comparison of Automated Quantitative Reverse Transcription-PCR and Direct Fluorescent-Antibody Detection for Routine Rabies Diagnosis in the United States.Journal of clinical microbiology · 2015Article
- Development of a duplex real-time RT-qPCR assay to monitor genome replication, gene expression and gene insert stability during in vivo replication of a prototype live attenuated canine distemper virus vector encoding SIV gag.Journal of virological methods · 2015Article
- Cross-platform evaluation of commercial real-time SYBR green RT-PCR kits for sensitive and rapid detection of European bat Lyssavirus type 1.BioMed research international · 2015Article
- Laboratory diagnosis of human rabies: recent advances.TheScientificWorldJournal · 2013Review
- Susceptibility and lack of evidence for a viremic state of rabies in the night owl monkey, Aotus nancymaae.Virology journal · 2012Article
- Improved PCR methods for detection of African rabies and rabies-related lyssaviruses.Journal of clinical microbiology · 2010Article
- Improved safety for molecular diagnosis of classical rabies viruses by use of a TaqMan real-time reverse transcription-PCR "double check" strategy.Journal of clinical microbiology · 2010Article
- Emerging technologies for the detection of rabies virus: challenges and hopes in the 21st century.PLoS neglected tropical diseases · 2009Article
- Lyssaviruses: current trends.Advances in virus research · 2008Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Published assays that use TaqMan PCR are consistently sensitive, rapid, and readily transferable. Here we describe a TaqMan PCR-based method for the detection of rabies virus (RV) RNA in tissue samples. We show that the method has an acceptable linear range, is both sensitive and specific, and, importantly, correlates with the concentration of infectious virus. In addition, the levels of RV-specific amplification are adjustable according to the levels of an endogenous control (beta-actin mRNA), allowing the calculation of comparable quantities. We tested the capacity of this assay to cope with target sequence variations. The number of sequence mismatches between gene-specific oligonucleotides and the target sequence significantly affects amplification (P < 0.001), and point mutations at the center of the probe can result in false-negative results through the prevention of probe binding and subsequent fluorescence. This study demonstrates that the genetic heterogeneity of RVs may prove a serious obstacle in the development of a diagnostic assay based on TaqMan PCR; however, the quantification of RV levels may prove to be a valuable application of this assay.
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