ArticleJournal of clinical microbiology2004
Validation of roche COBAS Amplicor assay for detection of Chlamydia trachomatis in rectal and pharyngeal specimens by an omp1 PCR assay.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- A cohort study of Chlamydia trachomatis treatment failure in women: a study protocol.BMC infectious diseases · 2013Trial
- Diagnosis of Chlamydia trachomatis genital infections in the era of genomic medicine.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2021Review
- Lymphogranuloma venereum: diagnostic and treatment challenges.Infection and drug resistance · 2015Review
- Analysis of laboratory testing results collected in an enhanced chlamydia surveillance system in Australia, 2008-2010.BMC infectious diseases · 2014Article
- Analytical evaluation of GeneXpert CT/NG, the first genetic point-of-care assay for simultaneous detection of Neisseria gonorrhoeae and Chlamydia trachomatis.Journal of clinical microbiology · 2013Article
- Differing Neisseria gonorrhoeae bacterial loads in the pharynx and rectum in men who have sex with men: implications for gonococcal detection, transmission, and control.Journal of clinical microbiology · 2011Article
- Asymptomatic sexually transmitted infections in HIV-infected men who have sex with men: prevalence, incidence, predictors, and screening strategies.AIDS patient care and STDs · 2008Article
- Confirming the Chlamydia trachomatis status of referred rectal specimens.Sexually transmitted infections · 2007Article
- Sexually transmitted proctitis.Postgraduate medical journal · 2006Review
- Testing specimens for Chlamydia trachomatis.Sexually transmitted infections · 2006Review
- The superiority of polymerase chain reaction over an amplified enzyme immunoassay for the detection of genital chlamydial infections.Sexually transmitted infections · 2006Article
- Is screening for sexually transmitted infections in men who have sex with men who receive non-occupational HIV post-exposure prophylaxis worthwhile?Sexually transmitted infections · 2006Article
- Molecular diagnosis of lymphogranuloma venereum: PCR-based restriction fragment length polymorphism and real-time PCR.Journal of clinical microbiology · 2005Article
- Chlamydia trachomatis serovars causing urogenital infections in women in Melbourne, Australia.Journal of clinical microbiology · 2005Article
- Variability of the Chlamydia trachomatis omp1 gene detected in samples from men tested in male-only saunas in Melbourne, Australia.Journal of clinical microbiology · 2004Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Screening guidelines for men who have sex with men (MSM) recommend testing of extragenital sites (pharyngeal and rectal) for gonorrhoea and chlamydia. Testing of specimens from these sites is not validated by most commercial nucleic amplification tests, such as the COBAS Amplicor assay. To investigate the utility of the COBAS Amplicor assay for detection of Chlamydia trachomatis in extragenital specimens, this study developed and evaluated confirmatory tests using the omp1 gene as an alternative target for amplification by PCR. Of anal and throat swabs collected from men in male-only saunas, 52 swabs that tested C. trachomatis positive by COBAS Amplicor and 30 swabs that tested as negative were included for confirmatory omp1 PCR testing. A total of 49 (94%) COBAS Amplicor-positive samples were confirmed by the omp1 PCR. A substantial proportion of specimens were confirmed by using a nested omp1 PCR (27%). Not confirmed by any omp1 PCR were three anal swabs (6%). It is most probable that these samples contained lower bacterial levels that were near or below the detection level of the omp1 PCR assays. The findings of this study support the confident reporting of C. trachomatis detected by COBAS Amplicor in extragenital specimens and support the utility of this assay as a screening test for MSM.
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