ArticleJournal of clinical microbiology2004
Detection of the Bacillus anthracis gyrA gene by using a minor groove binder probe.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Implications of limits of detection of various methods for Bacillus anthracis in computing risks to human health.Applied and environmental microbiology · 2009Pooled it
- Pan-genome analysis reveals novel chromosomal markers for multiplex PCR-based specific detection of Bacillus anthracis.BMC infectious diseases · 2024Article
- Methods of IdentifyingPathogens (Basel, Switzerland) · 2022Review
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- A Smartphone Integrated Platform for Ratiometric Fluorescent Sensitive and Selective Determination of Dipicolinic Acid.Biosensors · 2022Article
- Rapid Identification of Bacillus anthracisMicrobiology spectrum · 2022Article
- Development of a set of three real-time loop-mediated isothermal amplification (LAMP) assays for detection of Bacillus anthracis, the causative agent of anthrax.Folia microbiologica · 2021Article
- Molecular characterization of B. anthracis isolates from the anthrax outbreak among cattle in Karnataka, India.BMC microbiology · 2020Article
- The role of l-histidine as molecular tongs: a strategy of grasping TbChemical science · 2020Article
- Duplex Electrochemical DNA Sensor to DetectACS omega · 2019Article
- Review
- Holographic deep learning for rapid optical screening of anthrax spores.Science advances · 2017Article
- Bacillus cereus Biovar Anthracis Causing Anthrax in Sub-Saharan Africa-Chromosomal Monophyly and Broad Geographic Distribution.PLoS neglected tropical diseases · 2016Article
- Identification and validation of specific markers of Bacillus anthracis spores by proteomics and genomics approaches.Molecular & cellular proteomics : MCP · 2014Article
- In silico and in vitro evaluation of PCR-based assays for the detection of Bacillus anthracis chromosomal signature sequences.Virulence · 2013Article
- Discrimination of Bacillus anthracis and closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microarray.Chemico-biological interactions · 2008Article
- Development of a real-time fluorescence resonance energy transfer PCR to detect arcobacter species.Journal of clinical microbiology · 2007Article
- High throughput screening for spores and vegetative forms of pathogenic B. anthracis by an internally controlled real-time PCR assay with automated DNA preparation.Medical microbiology and immunology · 2007Article
- Simultaneous real-time PCR detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2007Article
- Tentacle probes: eliminating false positives without sacrificing sensitivity.Nucleic acids research · 2007Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identification of chromosomal markers for rapid detection of Bacillus anthracis is difficult because significant chromosomal homology exists among B. anthracis, Bacillus cereus, and Bacillus thuringiensis. We evaluated the bacterial gyrA gene as a potential chromosomal marker for B. anthracis. A real-time PCR assay was developed for the detection of B. anthracis. After analysis of the unique nucleotide sequence of the B. anthracis gyrA gene, a fluorescent 3' minor groove binding probe was tested with 171 organisms from 29 genera of bacteria, including 102 Bacillus strains. The assay was found to be specific for all 43 strains of B. anthracis tested. In addition, a test panel of 105 samples was analyzed to evaluate the potential diagnostic capability of the assay. The assay showed 100% specificity, demonstrating the usefulness of the gyrA gene as a specific chromosomal marker for B. anthracis.
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