ArticleJournal of clinical microbiology2004
Hepatitis C genotyping by denaturing high-performance liquid chromatography.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Selected hepatoprotective herbal medicines: Evidence from ethnomedicinal applications, animal models, and possible mechanism of actions.Phytotherapy research : PTR · 2018Review
- [Distribution of HCV genotypes in Chinese Han population with chronic hepatitis C].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2015Article
- Prevalence of hepatitis C virus genotypes in district bannu, khyber pakhtunkhwa, pakistan.Hepatitis research and treatment · 2014Article
- Changing pattern of clinical epidemiology on hepatitis C virus infection in southwest china.Hepatitis monthly · 2012Article
- HCV genotype distribution and possible transmission risks in Lahore, Pakistan.World journal of gastroenterology · 2010Article
- Molecular epidemiology of Hepatitis C virus genotypes in Khyber Pakhtoonkhaw of Pakistan.Virology journal · 2010Article
- Distribution of different hepatitis C virus genotypes in patients with hepatitis C virus infection.World journal of gastroenterology · 2010Article
- Nucleotide identity and variability among different Pakistani hepatitis C virus isolates.Virology journal · 2009Article
- HCV genotyping using statistical classification approach.Journal of biomedical science · 2009Article
- Frequency distribution of hepatitis C virus genotypes in different geographical regions of Pakistan and their possible routes of transmission.BMC infectious diseases · 2008Article
- Rapid genotyping of hepatitis C virus by primer-specific extension analysis.Journal of clinical microbiology · 2005Article
- Genotyping hepatitis C virus by heteroduplex mobility analysis using temperature gradient capillary electrophoresis.Journal of clinical microbiology · 2004Article
- Availability of pharmacogenomics-based prescribing information in drug package inserts for currently approved drugs.The pharmacogenomics journal · 2004Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Determination of the hepatitis C virus (HCV) genotype for infected patients increasingly has become accepted as the standard of care. Genotype assignment helps in assessing disease prognosis and assists in establishing the appropriate duration of treatment. The great genetic diversity of HCV, with 11 major genotypes and >70 subtypes, contributes to the technical difficulty of genotype testing. While the "gold standard" for testing is nucleic acid sequencing, a variety of hybridization assays, including the line probe assay, have been developed to provide more rapid and accessible forms of testing. The aim of this study was to determine whether denaturing high-performance liquid chromatography (dHPLC) could be used as a clinical method for distinguishing HCV genotypes 1, 2, 3, and 4. A portion of the 5' untranslated region of the HCV genome was amplified by heminested multiplex reverse transcription PCR. The two amplicons then were analyzed by dHPLC analysis and compared to the genotypes determined by sequence analysis. After 115 specimens were analyzed as standards, 200 masked specimens (specimens whose identity was not known before testing) were analyzed to determine the concordance of the assay. The assay had a concordance of 96% at the genotype level and a concordance of 87% at the subtype level. However, the dHPLC method was not as accurate as other reported methods of HCV genotyping. This is the first time that HCV genotyping has been performed by dHPLC.
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