ArticleJournal of clinical microbiology2004
Expression and immunogenicity of proteins encoded by sequences specific to Mycobacterium avium subsp. paratuberculosis.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Detection ofFrontiers in veterinary science · 2021Article
- Identification of Sero-Diagnostic Antigens for the Early Diagnosis of Johne's Disease using MAP Protein Microarrays.Scientific reports · 2019Article
- Early detection of Mycobacterium avium subsp. paratuberculosis infection in cattle with multiplex-bead based immunoassays.PloS one · 2017Article
- Antigenicity of recombinant maltose binding protein-Mycobacterium avium subsp. paratuberculosis fusion proteins with and without factor Xa cleaving.Clinical and vaccine immunology : CVI · 2013Article
- Several affinity tags commonly used in chromatographic purification.Journal of analytical methods in chemistry · 2013Review
- Serological evaluation of Mycobacterium ulcerans antigens identified by comparative genomics.PLoS neglected tropical diseases · 2010Article
- Identification and functional characterization of the iron-dependent regulator (IdeR) of Mycobacterium avium subsp. paratuberculosis.Microbiology (Reading, England) · 2009Article
- Genomic comparison of PE and PPE genes in the Mycobacterium avium complex.Journal of clinical microbiology · 2009Article
- Immunogenicity of proteome-determined Mycobacterium avium subsp. paratuberculosis-specific proteins in sheep with paratuberculosis.Clinical and vaccine immunology : CVI · 2008Article
- Profiling bovine antibody responses to Mycobacterium avium subsp. paratuberculosis infection by using protein arrays.Infection and immunity · 2008Article
- Early antibody response against Mycobacterium avium subspecies paratuberculosis antigens in subclinical cattle.Proteome science · 2008Article
- Genomics of Actinobacteria: tracing the evolutionary history of an ancient phylum.Microbiology and molecular biology reviews : MMBR · 2007Review
- Rapid expression of Mycobacterium avium subsp. paratuberculosis recombinant proteins for antigen discovery.Clinical and vaccine immunology : CVI · 2007Article
- The ability of Mycobacterium avium subsp. paratuberculosis to enter bovine epithelial cells is influenced by preexposure to a hyperosmolar environment and intracellular passage in bovine mammary epithelial cells.Infection and immunity · 2006Article
- Immunoglobulin G1 enzyme-linked immunosorbent assay for diagnosis of Johne's Disease in red deer (Cervus elaphus).Clinical and diagnostic laboratory immunology · 2005Article
- Expression library immunization confers protection against Mycobacterium avium subsp. paratuberculosis infection.Infection and immunity · 2005Article
- Development of a nested PCR method targeting a unique multicopy element, ISMap02, for detection of Mycobacterium avium subsp. paratuberculosis in fecal samples.Journal of clinical microbiology · 2005Article
- The complete genome sequence of Mycobacterium avium subspecies paratuberculosis.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Genomic polymorphisms for Mycobacterium avium subsp. paratuberculosis diagnostics.Journal of clinical microbiology · 2005Article
- Comparative genomic hybridizations reveal genetic regions within the Mycobacterium avium complex that are divergent from Mycobacterium avium subsp. paratuberculosis isolates.Journal of bacteriology · 2005Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of immunoassays specific for the diagnosis of Johne's disease in cattle requires antigens specific to Mycobacterium avium subsp. paratuberculosis. However, because of genetic similarity to other mycobacteria comprising the M. avium complex, no such antigens have been found. Through a comparative genomics approach, 21 potential coding sequences of M. avium subsp. paratuberculosis that are not represented in any other mycobacterial species tested (n = 9) were previously identified (J. P. Bannantine, E. Baechler, Q. Zhang, L. Li, and V. Kapur, J. Clin. Microbiol. 40:1303-1310, 2002). Here we describe the cloning, heterologous expression, and antigenic analysis of these M. avium subsp. paratuberculosis-specific sequences in Escherichia coli. Nucleotide sequences representing each unique predicted coding region were amplified and cloned into two different E. coli expression vectors encoding polyhistidine or maltose binding protein (MBP) affinity purification tags. All 21 of the MBP fusion proteins were successfully purified under denaturing conditions and were evaluated in immunoblotting studies with sera from rabbits and mice immunized with M. avium subsp. paratuberculosis. These studies showed that 5 of the 21 gene products are produced by M. avium subsp. paratuberculosis and are antigenic. Immunoblot analysis with a panel of sera from 9 healthy cattle and 10 cattle with clinical disease shows that the same five M. avium subsp. paratuberculosis proteins are also detected within the context of infection. Collectively, these studies have used a genomic approach to identify novel M. avium subsp. paratuberculosis antigens that are not present in any other mycobacteria. These findings may have a major impact on improved diagnostics for Johne's disease.
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