ArticleClinical and diagnostic laboratory immunology2004
Antibody reactivity to Omp31 from Brucella melitensis in human and animal infections by smooth and rough Brucellae.
Article in Clinical and diagnostic laboratory immunology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 61 citations in OpenAlex.
- A fluorescent bead-based multiplex assay for the detection ofFrontiers in microbiology · 2025Article
- Immunoinformatics analysis of Brucella melitensis to approach a suitable vaccine against brucellosis.Journal, genetic engineering & biotechnology · 2023Article
- Brucellosis detection and the role ofVeterinary world · 2023Review
- Comparison of BP26, Omp25 and Omp31 and a Multiepitope-Based Fusion Protein in the Serological Detection of Canine Brucellosis.Infection and drug resistance · 2022Article
- Evaluation of chimeric proteins for serological diagnosis of brucellosis in cattle.Veterinary world · 2021Article
- Seroprevalence of Brucellosis in goats in some selected areas of Bangladesh.Journal of advanced veterinary and animal research · 2021Article
- Efficient Immunization of BALB/c Mice against Pathogenic Brucella melitensis andIranian journal of biotechnology · 2021Article
- Diagnosis of human and canineHeliyon · 2020Article
- Use of recombinantVeterinary world · 2020Article
- Diagnostic potential ofCentral-European journal of immunology · 2018Article
- Characterization of novel Omp31 antigenic epitopes of Brucella melitensis by monoclonal antibodies.BMC microbiology · 2017Article
- Immunoreactivity evaluation of a new recombinant chimeric protein againstIranian journal of microbiology · 2016Article
- Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein.Journal of veterinary science · 2016Article
- Immune reactivity of sera obtained from brucellosis patients and vaccinated-rabbits to a fusion protein from Brucella melitensis.Iranian journal of basic medical sciences · 2015Article
- Evaluation of the efficacy of outer membrane protein 31 vaccine formulations for protection against Brucella canis in BALB/c mice.Clinical and vaccine immunology : CVI · 2014Article
- In silico analysis of chimeric TF, Omp31 and BP26 fragments of Brucella melitensis for development of a multi subunit vaccine candidate.Iranian journal of basic medical sciences · 2014Article
- Immune reactivity of Brucella melitensis-vaccinated rabbit serum with recombinant Omp31 and DnaK proteins.Iranian journal of microbiology · 2013Article
- 16S rRNA and omp31 gene based molecular characterization of field strains of B. melitensis from aborted foetus of goats in India.TheScientificWorldJournal · 2013Article
- Enzymatic and biological characteristics of enolase in Brucella abortus A19.Molecular biology reports · 2012Article
- Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens.PLoS neglected tropical diseases · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Group 3 of outer membrane proteins (OMPs) of Brucella includes Omp25 and Omp31, which share 34% identity. Omp25 is highly conserved in Brucella species, and Omp31 is present in all Brucella species, except Brucella abortus. Antibodies to Brucella melitensis Omp31 have been sought only in infected sheep, and Western blotting of sera from infected sheep did not reveal anti-Omp31 reactivity. We obtained recombinant purified Omp31 (B. melitensis) and tested its recognition by sera from humans and animals suffering from brucellosis by an indirect enzyme-linked immunosorbent assay (ELISA). Serum samples from 74 patients, 57 sheep, and 47 dogs were analyzed; brucellosis was confirmed by bacteriological isolation in all ovine and canine cases and 31 human cases of brucellosis. Thirty-five patients (47%) were positive for antibodies to Omp31, including seven cases of Brucella suis infection, two cases of B. abortus infection, and three cases of B. melitensis infection. Of 39 sheep naturally infected with B. melitensis (biovars 1 and 3), 23 (59%) were positive for antibodies to Omp31. Anti-Omp31 antibodies were also detected in 12 of 18 rams (67%) in which Brucella ovis was isolated from semen. Antibodies to Omp31 were also found in 41 (87%) of the 47 dogs, including 13 with recent infection. These results suggest that an indirect ELISA using recombinant purified Omp31 from B. melitensis would be of limited value for the diagnosis of human and animal brucellosis. Nevertheless, the potential usefulness of this antigen in combination with other recombinant proteins from Brucella should not be dismissed.
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