ArticleClinical and diagnostic laboratory immunology2004
Measurement of serum bactericidal activity specific for Haemophilus influenzae type b by using a chromogenic and fluorescent metabolic indicator.
Article in Clinical and diagnostic laboratory immunology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Trial
- A Bioluminescence-Based Serum Bactericidal Assay to Detect Bactericidal Antibodies AgainstMicroorganisms · 2025Article
- Functional assays to evaluate antibody-mediated responses againstFrontiers in cellular and infection microbiology · 2023Review
- A Novel Luminescence-Based Serum Bactericidal Assay for Vibrio cholerae Reduces Assay Variation, Is Time- and Cost-Effective, and Directly Measures Continuous Titer Values.The American journal of tropical medicine and hygiene · 2021Article
- Recognition of conserved antigens by Th17 cells provides broad protection against pulmonaryProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Development of a high-throughput method to evaluate serum bactericidal activity using bacterial ATP measurement as survival readout.PloS one · 2017Article
- A high throughput serum bactericidal assay for antibodies to Haemophilus influenzae type b.BMC infectious diseases · 2016Article
- A capsule conjugate vaccine approach to prevent diarrheal disease caused by Campylobacter jejuni.Human vaccines & immunotherapeutics · 2014Review
- Risk of invasive Haemophilus influenzae type b (Hib) disease in adults with secondary immunodeficiency in the post-Hib vaccine era.Clinical and vaccine immunology : CVI · 2012Article
- Development of an automated, high-throughput bactericidal assay that measures cellular respiration as a survival readout for Neisseria meningitidis.Clinical and vaccine immunology : CVI · 2011Article
- Evaluation of serum bactericidal antibody assays for Haemophilus influenzae serotype a.Clinical and vaccine immunology : CVI · 2011Article
- Immunogenicity and safety of two different Haemophilus influenzae type b conjugate vaccines in Korean infants.Journal of Korean medical science · 2008Article
- Fluorescent multivalent opsonophagocytic assay for measurement of functional antibodies to Streptococcus pneumoniae.Clinical and diagnostic laboratory immunology · 2005Article
- Avidity determinations for Haemophilus influenzae Type b anti-polyribosylribitol phosphate antibodies.Clinical and diagnostic laboratory immunology · 2005Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We evaluated alamarBlue as a metabolic indicator in a standardized assay for the measurement of serum bactericidal activity (SBA) to Haemophilus influenzae type b (Hib) using sera containing natural and vaccine-induced anticapsular (polyribosylribitol phosphate) antibodies. SBA assays with a colorimetric and a fluorometric end point in the presence of alamarBlue were developed and compared to a standard SBA assay, where colony counts are performed to determine the titer (12). A colorimetric end point required a spectrophotometer, whereas a fluorometric end point required a fluorometer. Prevaccination sera (n = 27) and postvaccination sera (n = 13) were tested by all three methodologies, and the SBA titers obtained in the presence of alamarBlue were compared to those from the standard method. Both the colorimetric and the fluorometric SBA titers were significantly correlated (r = 0.87 and r = 0.95, respectively) with those of the standard assay (>/= 50% killing as the SBA titer end point), and titers were not significantly different when compared to those of the standard assay (P > 0.68). However, the fluorometric end point had superior performance and ease of titer determination compared to the colorimetric end point (95 versus 87% of SBA titers were within 2 dilutions of the standard titer). Hib SBA assays with alamarBlue are reproducible, faster (same-day assay), and easier to perform than the standardized assay, which requires manual or automated colony counts. These semiautomated methodologies result in increased sample throughput and collection of data in digital formats that can be exported to data analysis programs for determination of SBA titers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.