ArticleBiochemical and biophysical research communications2004
A two-component signal transduction system with a PAS domain-containing sensor is required for virulence of Mycobacterium tuberculosis in mice.
Article in Biochemical and biophysical research communications, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 74 citations in OpenAlex.
- RegX3-Mediated Regulation of Methylcitrate Cycle inFrontiers in microbiology · 2021Article
- Dual control of RegX3 transcriptional activity by SenX3 and PknB.The Journal of biological chemistry · 2019Article
- Host-pathogen redox dynamics modulate Mycobacterium tuberculosis pathogenesis.Pathogens and disease · 2018Article
- Regulation of the CRISPR-Associated Genes by Rv2837c (CnpB) via an Orn-Like Activity in Tuberculosis Complex Mycobacteria.Journal of bacteriology · 2018Article
- The emerging role of gasotransmitters in the pathogenesis of tuberculosis.Nitric oxide : biology and chemistry · 2016Review
- Mycobacterium tuberculosis Transcription Machinery: Ready To Respond to Host Attacks.Journal of bacteriology · 2016Review
- Virulence factor SenX3 is the oxygen-controlled replication switch of Mycobacterium tuberculosis.Antioxidants & redox signaling · 2015Article
- Reprogramming of Yersinia from virulent to persistent mode revealed by complex in vivo RNA-seq analysis.PLoS pathogens · 2015Article
- senX3-independent contribution of regX3 to Mycobacterium tuberculosis virulence.BMC microbiology · 2014Article
- Differential regulation of the two-component regulatory system senX3-regX3 in Mycobacterium tuberculosis.Microbiology (Reading, England) · 2014Article
- Reductive evolution and the loss of PDC/PAS domains from the genus Staphylococcus.BMC genomics · 2013Article
- Virulence factors of the Mycobacterium tuberculosis complex.Virulence · 2013Review
- A genome-wide study of two-component signal transduction systems in eight newly sequenced mutans streptococci strains.BMC genomics · 2012Article
- Article
- The β-propeller gene Rv1057 of Mycobacterium tuberculosis has a complex promoter directly regulated by both the MprAB and TrcRS two-component systems.Tuberculosis (Edinburgh, Scotland) · 2011Article
- Adaptation to environmental stimuli within the host: two-component signal transduction systems of Mycobacterium tuberculosis.Microbiology and molecular biology reviews : MMBR · 2011Review
- The biological and structural characterization of Mycobacterium tuberculosis UvrA provides novel insights into its mechanism of action.Nucleic acids research · 2011Article
- Control of CydB and GltA1 expression by the SenX3 RegX3 two component regulatory system of Mycobacterium tuberculosis.PloS one · 2011Article
- Is Mycobacterium tuberculosis stressed out? A critical assessment of the genetic evidence.Microbes and infection · 2010Review
- Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Mycobacterium tuberculosis, the causative organism of tuberculosis, encounters oxidative stress during phagocytosis by the macrophage and following macrophage activation during an acquired immune response, and also from internally generated sources of radical oxygen intermediates through intermediary metabolism. We have identified the SenX3 protein, a sensor in 1 of the 11 complete pairs of two-component signal transduction systems in M. tuberculosis, as a possible orthologue of the Mak2p protein from the fission yeast Schizosaccharomyces pombe that is known to sense peroxide stress. Moreover, the SenX3-RegX3 two-component system was the top scoring hit in a homology search with the Escherichia coli ArcB-ArcA global control system of aerobic genes. Using structural modelling techniques we have determined that SenX3 contains a PAS-like domain found in a variety of prokaryotic and eukaryotic sensors of oxygen and redox. Mutants with knock-outs of senX3 or of the accompanying transcriptional regulator regX3 were constructed and found to have reduced virulence in a mouse model of tuberculosis infection, the mutant bacteria persisting for up to 4 months post-infection; complemented mutants had regained virulence confirming that it was mutations of this two-component system that were responsible for the avirulent phenotype. This work identifies the PAS domain as a possible drug target for tuberculosis and mutations in the senX3-regX signal transduction system as potentially useful components of live vaccine strains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.