ArticleEuropean biophysics journal : EBJ2004
Modeling of basolateral ATP release induced by hypotonic treatment in A6 cells.
Article in European biophysics journal : EBJ, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Cell culture: complications due to mechanical release of ATP and activation of purinoceptors.Cell and tissue research · 2017Review
- Purinergic signalling in the kidney in health and disease.Purinergic signalling · 2014Review
- Homeostasis of extracellular ATP in human erythrocytes.The Journal of biological chemistry · 2011Article
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Authors and funding
2 authors.
Funding
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Abstract
ATP is released from the basolateral membrane of A6 epithelia in response to hypotonic treatment. This study addresses the problem of ATP diffusion through the permeable supports used to culture the cells. A theoretical analysis of a recently introduced experimental protocol is presented and a model of ATP diffusion through the compartments of the measuring system is proposed. The model provides the ATP profiles near the cell layer and in the measurement chamber. Comparison of results from computer simulations and experimental data showed that the permeable support introduces a marked delay for ATP diffusion, supporting the correlation of apparently time-separated events: the mobilization of Ca(2+) from internal stores and release of ATP from the cell. The model is consistent with experimental data obtained with the luciferin-luciferase pulse protocol and provides an indirect proof of related processes like the closure and opening of the lateral interspace that occur after imposing the hyposmotic shock. The influence of the pore structure of the permeable support in modulating the measured release rates revealed by computer simulation is experimentally validated for two types of Anopore filters.
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Registered trials
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