ArticleThe EMBO journal2004
Coupling of caspase-9 to Apaf1 in response to loss of pRb or cytotoxic drugs is cell-type-specific.
Article in The EMBO journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Non-apoptotic role of caspase enzymes in satellite cells from human skeletal muscle.Biogerontology · 2026Article
- Bridging Inflammation and Repair: The Promise of MFG-E8 in Ischemic Stroke Therapy.International journal of molecular sciences · 2025Review
- The tumor suppressor pRb and its relative p130 are required to maintain murine adult skeletal muscle homeostasis.Oncogene · 2025Article
- IRE1α promotes viral infection by conferring resistance to apoptosis.Science signaling · 2017Article
- Apoptosis in differentiating C2C12 muscle cells selectively targets Bcl-2-deficient myotubes.Apoptosis : an international journal on programmed cell death · 2014Article
- Dietary fat modifies mitochondrial and plasma membrane apoptotic signaling in skeletal muscle of calorie-restricted mice.Age (Dordrecht, Netherlands) · 2013Article
- The impairment of HCCS leads to MLS syndrome by activating a non-canonical cell death pathway in the brain and eyes.EMBO molecular medicine · 2013Article
- Apoptotic-induced cleavage shifts HuR from being a promoter of survival to an activator of caspase-mediated apoptosis.Cell death and differentiation · 2013Article
- Rb1 gene inactivation expands satellite cell and postnatal myoblast pools.The Journal of biological chemistry · 2011Article
- Article
- Rescue of myogenic defects in Rb-deficient cells by inhibition of autophagy or by hypoxia-induced glycolytic shift.The Journal of cell biology · 2010Article
- Mitochondrial abnormalities in spinal and bulbar muscular atrophy.Human molecular genetics · 2009Article
- Skeletal muscle differentiation evokes endogenous XIAP to restrict the apoptotic pathway.PloS one · 2009Article
- Apoptosome-deficient cells lose cytochrome c through proteasomal degradation but survive by autophagy-dependent glycolysis.Molecular biology of the cell · 2008Article
- BNIP3 is an RB/E2F target gene required for hypoxia-induced autophagy.Molecular and cellular biology · 2007Article
- XIAP activity dictates Apaf-1 dependency for caspase 9 activation.Molecular and cellular biology · 2007Article
- Loss of caspase-9 reveals its essential role for caspase-2 activation and mitochondrial membrane depolarization.Molecular biology of the cell · 2007Article
- Functional identification of Api5 as a suppressor of E2F-dependent apoptosis in vivo.PLoS genetics · 2006Article
- Apoptosome-independent activation of the lysosomal cell death pathway by caspase-9.Molecular and cellular biology · 2006Article
- Molecular regulation of apoptosis in fast plantaris muscles of aged rats.The journals of gerontology. Series A, Biological sciences and medical sciences · 2006Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inactivation of the tumor suppressor Rb in the mouse induces cell death, which depends entirely (in lens, CNS) and only partly (PNS, skeletal muscles) on Apaf1/Ced4, an apoptosomal factor thought to be required for processing procaspase-9 following mitochondrial permeabilization. Here, we report that in response to cytotoxic drugs, Apaf1(-/-) primary myoblasts but not fibroblasts undergo bona fide apoptosis. Cell demise was associated with disruption of mitochondria but not endoplasmic reticulum. Processing of procaspase-9 occurred in Apaf1(-/-) myoblasts but not fibroblasts, and ablation of Casp9 prevented drug-induced apoptosis in both cell types. Deregulation of the Rb pathway by overexpression of E2F1 also induced caspase-9-dependent, Apaf1-independent apoptosis in myoblasts. Despite its requirement for apoptosis in vitro, mutation in Casp9 abrogated cell death in the nervous system and lens but only partly in skeletal muscles of Rb-deficient embryos. In addition, developmental cell death in fetal liver and PNS was not inhibited in Casp9(-/-) embryos. Therefore, loss of pRb elicits apoptosome-dependent and apoptosome-independent cell death, and the requirement and coupling of caspase-9 to Apaf1 are both context-dependent.
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