ArticleApplied and environmental microbiology2004
Biodegradation of nitro-substituted explosives 2,4,6-trinitrotoluene, hexahydro-1,3,5-trinitro-1,3,5-triazine, and octahydro-1,3,5,7-tetranitro-1,3,5-tetrazocine by a phytosymbiotic Methylobacterium sp. associated with poplar tissues (Populus deltoides x nigra DN34).
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 264 citations in OpenAlex.
- Pink powerhouses: insights into the multifaceted role of Methylobacterium in climate-resilient farming.Folia microbiologica · 2025Review
- Treatment of Xenobiotic Cyclic Nitramine Explosives in Wastewater.Journal of xenobiotics · 2025Review
- Biopriming with Endophytes improves plant resilience to develop climate-smart, futuristic agricultural crops.World journal of microbiology & biotechnology · 2025Review
- Impact of glyphosate and glyphosate-based herbicides on phyllospheric Methylobacterium.BMC plant biology · 2024Article
- Microbiomes of urban trees: unveiling contributions to atmospheric pollution mitigation.Frontiers in microbiology · 2024Article
- Trends in Harnessing Plant Endophytic Microbiome for Heavy Metal Mitigation in Plants: A Perspective.Plants (Basel, Switzerland) · 2023Review
- Phytoremediation, Bioaugmentation, and the Plant Microbiome.Environmental science & technology · 2022Review
- An Overview of Treatment Approaches for Octahydro-1, 3, 5, 7-tetranitro-1, 3, 5, 7-tetrazocine (HMX) Explosive in Soil, Groundwater, and Wastewater.International journal of environmental research and public health · 2022Review
- A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones.BMC microbiology · 2022Article
- Degradation of 2,4,6-Trinitrotoluene (TNT): Involvement of Protocatechuate 3,4-Dioxygenase (P34O) inToxics · 2021Article
- Harnessing Bacterial Endophytes for Promotion of Plant Growth and Biotechnological Applications: An Overview.Plants (Basel, Switzerland) · 2021Review
- The Response of the Associations of Grass andPlants (Basel, Switzerland) · 2021Review
- First report ofNew microbes and new infections · 2019Article
- Aerobic biodegradation of HMX by3 Biotech · 2018Article
- Belowground Microbiota and the Health of Tree Crops.Frontiers in microbiology · 2018Review
- Biotransformation of 2,4-dinitroanisole by a fungal Penicillium sp.Biodegradation · 2017Article
- Plant-bacteria partnerships for the remediation of persistent organic pollutants.Environmental science and pollution research international · 2017Article
- Phylloremediation of Air Pollutants: Exploiting the Potential of Plant Leaves and Leaf-Associated Microbes.Frontiers in plant science · 2017Review
- Effects of Secondary Plant Metabolites on Microbial Populations: Changes in Community Structure and Metabolic Activity in Contaminated Environments.International journal of molecular sciences · 2016Review
- Uptake of carbamazepine by rhizomes and endophytic bacteria of Phragmites australis.Frontiers in plant science · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A pink-pigmented symbiotic bacterium was isolated from hybrid poplar tissues (Populus deltoides x nigra DN34). The bacterium was identified by 16S and 16S-23S intergenic spacer ribosomal DNA analysis as a Methylobacterium sp. (strain BJ001). The isolated bacterium was able to use methanol as the sole source of carbon and energy, which is a specific attribute of the genus Methylobacterium. The bacterium in pure culture was shown to degrade the toxic explosives 2,4,6-trinitrotoluene (TNT), hexahydro-1,3,5-trinitro-1,3,5-triazene (RDX), and octahydro-1,3,5,7-tetranitro-1,3,5-tetrazocine (HMX). [U-ring-(14)C]TNT (25 mg liter(-1)) was fully transformed in less than 10 days. Metabolites included the reduction derivatives amino-dinitrotoluenes and diamino-nitrotoluenes. No significant release of (14)CO(2) was recorded from [(14)C]TNT. In addition, the isolated methylotroph was shown to transform [U-(14)C]RDX (20 mg liter(-1)) and [U-(14)C]HMX (2.5 mg liter(-1)) in less than 40 days. After 55 days of incubation, 58.0% of initial [(14)C]RDX and 61.4% of initial [(14)C]HMX were mineralized into (14)CO(2). The radioactivity remaining in solution accounted for 12.8 and 12.7% of initial [(14)C]RDX and [(14)C]HMX, respectively. Metabolites detected from RDX transformation included a mononitroso RDX derivative and a polar compound tentatively identified as methylenedinitramine. Since members of the genus Methylobacterium are distributed in a wide diversity of natural environments and are very often associated with plants, Methylobacterium sp. strain BJ001 may be involved in natural attenuation or in situ biodegradation (including phytoremediation) of explosive-contaminated sites.
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