ArticleApplied and environmental microbiology2004
Molecular cloning and expression in Saccharomyces cerevisiae of a laccase gene from the ascomycete Melanocarpus albomyces.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 90 citations in OpenAlex.
- Decoding the distribution, structure-function-redox potential relationship and recent advances in fungal laccases: a systematic approach.Bioprocess and biosystems engineering · 2026Review
- Optimisation of the Production and Bleaching Process for a New Laccase from Madurella mycetomatis, Expressed in Pichia pastoris: from Secretion to Yielding Prominent.Molecular biotechnology · 2021Article
- Phenoloxidases in Plants-How Structural Diversity Enables Functional Specificity.Frontiers in plant science · 2021Review
- Aptitude of Oxidative Enzymes for Treatment of Wastewater Pollutants: A Laccase Perspective.Molecules (Basel, Switzerland) · 2019Review
- Expression of a new laccase from Moniliophthora roreri at high levels in Pichia pastoris and its potential application in micropollutant degradation.AMB Express · 2017Article
- Biochemical properties and yields of diverse bacterial laccase-like multicopper oxidases expressed in Escherichia coli.Scientific reports · 2015Article
- Diversity of laccase-coding genes in Fusarium oxysporum genomes.Frontiers in microbiology · 2015Article
- Molecular adaptation mechanisms employed by ethanologenic bacteria in response to lignocellulose-derived inhibitory compounds.International journal of biological sciences · 2013Review
- Enhanced archaeal laccase production in recombinant Escherichia coli by modification of N-terminal propeptide and twin arginine translocation motifs.Journal of industrial microbiology & biotechnology · 2012Article
- The Aspergillus niger multicopper oxidase family: analysis and overexpression of laccase-like encoding genes.Microbial cell factories · 2011Article
- Heterologous expression of a tannic acid-inducible laccase3 of Cryphonectria parasitica in Saccharomyces cerevisiae.BMC biotechnology · 2010Article
- Laccases: a never-ending story.Cellular and molecular life sciences : CMLS · 2010Review
- Transgenic rice as a novel production system for Melanocarpus and Pycnoporus laccases.Transgenic research · 2008Article
- Physiological evaluation of the filamentous fungus Trichoderma reesei in production processes by marker gene expression analysis.BMC biotechnology · 2007Article
- Heterologous laccase production and its role in industrial applications.Bioengineered bugsReview
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lac1 gene encoding an extracellular laccase was isolated from the thermophilic fungus Melanocarpus albomyces. This gene has five introns, and it encodes a protein consisting of 623 amino acids. The deduced amino acid sequence of the laccase was shown to have high homology with laccases from other ascomycetes. In addition to removal of a putative 22-amino-acid signal sequence and a 28-residue propeptide, maturation of the translation product of lac1 was shown to involve cleavage of a C-terminal 14-amino-acid extension. M. albomyces lac1 cDNA was expressed in Saccharomyces cerevisiae under the inducible GAL1 promoter. Extremely low production was obtained with the expression construct containing laccase cDNA with its own signal and propeptide sequences. The activity levels were significantly improved by replacing these sequences with the prepro sequence of the S. cerevisiae alpha-factor gene. The role of the C-terminal extension in laccase production in S. cerevisiae was also studied. Laccase production was increased sixfold with the modified cDNA that had a stop codon after the native processing site at the C terminus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.