ArticleApplied and environmental microbiology2004
Rapid and specific detection of Salmonella spp. in animal feed samples by PCR after culture enrichment.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 93 citations in OpenAlex.
- Advances in detection methods for viable Salmonella spp.: current applications and challenges.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023Review
- Determination of frequency, multiple antibiotic resistance index and resistotype of Salmonella spp. in chicken meat collected from southeast of Iran.Veterinary medicine and science · 2022Article
- Selected Instrumental Techniques Applied in Food and Feed: Quality, Safety and Adulteration Analysis.Foods (Basel, Switzerland) · 2021Review
- A molecule capturer analysis system for visual determination of avian pathogenic Escherichia coli serotype O78 using a lateral flow assay.Mikrochimica acta · 2020Article
- Detection ofBiosensors · 2019Article
- Evaluation of Contamination Risks with Coxsackievirus B4 E2 in Swiss Albino Mice Stools.Current microbiology · 2018Article
- Comparative evaluation of two Rickettsia typhi-specific quantitative real-time PCRs for research and diagnostic purposes.Medical microbiology and immunology · 2017Article
- Evaluation of a multiplex PCR method to serotypeMethodsX · 2017Article
- Screening and DetectingFrontiers in microbiology · 2017Article
- A high-sensitivity optical device for the early monitoring of plant pathogen attack via the in vivo detection of ROS bursts.Frontiers in plant science · 2015Article
- Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples.BMC microbiology · 2008Article
- Quantification of Campylobacter spp. in chicken rinse samples by using flotation prior to real-time PCR.Applied and environmental microbiology · 2005Article
- Toward standardization of diagnostic PCR testing of fecal samples: lessons from the detection of salmonellae in pigs.Journal of clinical microbiology · 2005Review
- Species-specific detection and quantification of toxic marine dinoflagellates Alexandrium tamarense and A. catenella by Real-time PCR assay.Marine biotechnology (New York, N.Y.)Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A PCR procedure has been developed for routine analysis of viable Salmonella spp. in feed samples. The objective was to develop a simple PCR-compatible enrichment procedure to enable DNA amplification without any sample pretreatment such as DNA extraction or cell lysis. PCR inhibition by 14 different feed samples and natural background flora was circumvented by the use of the DNA polymerase Tth. This DNA polymerase was found to exhibit a high level of resistance to PCR inhibitors present in these feed samples compared to DyNAzyme II, FastStart Taq, Platinum Taq, Pwo, rTth, Taq, and Tfl. The specificity of the Tth assay was confirmed by testing 101 Salmonella and 43 non-Salmonella strains isolated from feed and food samples. A sample preparation method based on culture enrichment in buffered peptone water and DNA amplification with Tth DNA polymerase was developed. The probability of detecting small numbers of salmonellae in feed, in the presence of natural background flora, was accurately determined and found to follow a logistic regression model. From this model, the probability of detecting 1 CFU per 25 g of feed in artificially contaminated soy samples was calculated and found to be 0.81. The PCR protocol was evaluated on 155 naturally contaminated feed samples and compared to an established culture-based method, NMKL-71. Eight percent of the samples were positive by PCR, compared with 3% with the conventional method. The reasons for the differences in sensitivity are discussed. Use of this method in the routine analysis of animal feed samples would improve safety in the food chain.
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