ArticleBMC physiology2004
Heterotrimeric G protein subunits are located on rat liver endosomes.
Article in BMC physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
- Overexpression of a Novel Noxo1 Mutant Increases Ros Production and Noxo1 Relocalisation.International journal of molecular sciences · 2023Article
- Glucagon's Metabolic Action in Health and Disease.Comprehensive Physiology · 2021Article
- Components of the GTraffic (Copenhagen, Denmark) · 2020Article
- Gαs regulates Glucagon-Like Peptide 1 Receptor-mediated cyclic AMP generation at Rab5 endosomal compartment.Molecular metabolism · 2017Article
- ARF6 Is an Actionable Node that Orchestrates Oncogenic GNAQ Signaling in Uveal Melanoma.Cancer cell · 2016Article
- Heterotrimeric G protein-mediated signaling and its non-canonical regulation in the heart.Life sciences · 2015Review
- Cadherin-6B undergoes macropinocytosis and clathrin-mediated endocytosis during cranial neural crest cell EMT.Journal of cell science · 2015Article
- Gαs regulates the post-endocytic sorting of G protein-coupled receptors.Nature communications · 2014Article
- Nuclear Na+/K+-ATPase plays an active role in nucleoplasmic Ca2+ homeostasis.Journal of cell science · 2012Article
- G protein trafficking.Sub-cellular biochemistry · 2012Review
- Non-canonical signaling and localizations of heterotrimeric G proteins.Cellular signalling · 2012Review
- Shuttling and translocation of heterotrimeric G proteins and Ras.Trends in pharmacological sciences · 2009Review
- Regulation of the V-ATPase along the endocytic pathway occurs through reversible subunit association and membrane localization.PloS one · 2008Article
- A specific role of AGS3 in the surface expression of plasma membrane proteins.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- An obligatory requirement for the heterotrimeric G protein Gi3 in the antiautophagic action of insulin in the liver.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Regulation of epidermal growth factor receptor degradation by heterotrimeric Galphas protein.Molecular biology of the cell · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
backgroundRat liver endosomes contain activated insulin receptors and downstream signal transduction molecules. We undertook these studies to determine whether endosomes also contain heterotrimeric G proteins that may be involved in signal transduction from G protein-coupled receptors.
resultsBy Western blotting Gsalpha, Gialpha1,2, Gialpha3 and Gbeta were enriched in both canalicular (CM) and basolateral (BLM) membranes but also readily detectable on three types of purified rat liver endosomes in the order recycling receptor compartment (RRC) > compartment for uncoupling of receptor and ligand (CURL) > multivesicular bodies (MVB) >> purified secondary lysosomes. Western blotting with antibodies to Na, K-ATPase and to other proteins associated with plasma membranes and intracellular organelles indicated this was not due to contamination of endosome preparations by CM or BLM. Adenylate cyclase (AC) was also identified on purified CM, BLM, RRC, CURL and MVB. Percoll gradient fractionation of liver postnuclear supernatants demonstrated co-occurrence of endosomes and heterotrimeric G protein subunits in fractions with little plasma membrane markers. By confocal microscopy, punctate staining for Gsalpha, Gialpha3 and Gbeta corresponded to punctate areas of endocytosed Texas red-dextran in hepatocytes from control and cholera toxin-treated livers.
conclusionWe conclude that heterotrimeric G protein subunits as well as AC likely traffic into hepatocytes on endosome membranes, possibly generating downstream signals spatially separate from signalling generated at the plasma membrane, analogous to the role(s) of internalized insulin receptors.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.