ArticleProceedings of the National Academy of Sciences of the United States of America2004
A proteomic approach for the analysis of instantly released wound and immune proteins in Drosophila melanogaster hemolymph.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 150 citations in OpenAlex.
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- Hemolymph protein profiles of subterranean termite Reticulitermes flavipes challenged with methicillin resistant Staphylococcus aureus or Pseudomonas aeruginosa.Scientific reports · 2018Article
- Understanding Leishmania parasites through proteomics and implications for the clinic.Expert review of proteomics · 2018Review
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- BgTEP: An Antiprotease Involved in Innate Immune Sensing inFrontiers in immunology · 2018Article
- Proteomic profile of the Bradysia odoriphaga in response to the microbial secondary metabolite benzothiazole.Scientific reports · 2016Article
- Actin is an evolutionarily-conserved damage-associated molecular pattern that signals tissue injury ineLife · 2016Article
- Cytoplasmic actin is an extracellular insect immune factor which is secreted upon immune challenge and mediates phagocytosis and direct killing of bacteria, and is a Plasmodium Antagonist.PLoS pathogens · 2015Article
- Differential gene expression and alternative splicing in insect immune specificity.BMC genomics · 2014Article
- Lifestyle and host defense mechanisms of the dung beetle, Euoniticellus intermedius: the toll signaling pathway.Journal of insect science (Online) · 2013Article
- The hemolymph proteome of fed and starved Drosophila larvae.PloS one · 2013Article
- Proteomic biomarkers for ageing the mosquito Aedes aegypti to determine risk of pathogen transmission.PloS one · 2013Article
- Adaptive evolution of a novel Drosophila lectin induced by parasitic wasp attack.Molecular biology and evolution · 2012Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insects respond to microbial infection by the rapid and transient expression of several genes encoding antibacterial peptides. In this paper we describe a powerful technique, two-dimensional difference gel electrophoresis, that, when combined with mass spectrometry, can be used to study the immune response of Drosophila melanogaster at the protein level. By comparatively analyzing the hemolymph proteome of 2,000 third-instar Drosophila larvae, we identified 10 differential proteins that appear in the fruit fly hemolymph very early after an immune-challenge with lipopolysaccharides. These proteins can be assigned to the immune response, because they are not induced after sterile injury. Reduction of integral variability or quantification problems related to conventional two-dimensional electrophoresis and improvement of image analysis were achieved by the use of two fluorescent dyes to label the two different protein samples. Some of the immune-induced proteins, such as thioester-containing protein 2, can be assigned to specific aspects of the immune response; others were already reported as being involved in stress response. An immune-induced protein (CG18594) is homologous to a mammalian serine protease inhibitor that mediates the mitogen-activated protein kinase and the NF-kappa B signaling pathways. In addition, a number of proteins that had not been associated with the immune response before were isolated and identified, and some of these were still present in the hemolymph 4 h after injury. Determining the function of all of these immune-induced proteins represents an exciting challenge for increasing our knowledge of insect immunity.
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