ArticleGenome research2004
High-throughput MALDI-TOF discovery of genomic sequence polymorphisms.
Article in Genome research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed.
- Randomized Phase 2 Trial of Pharmacodynamic Separation of Pemetrexed and Intercalated Erlotinib Versus Pemetrexed Alone for Advanced Nonsquamous, Non-small-cell Lung Cancer.Clinical lung cancer · 2017Trial
- Design and validation of a clinical whole genome sequencing-based assay for patient screening in a large healthcare system.Frontiers in molecular biosciences · 2025Article
- Visualisation of Host-Pathogen Communication.Advances in experimental medicine and biology · 2023Article
- Single step multiple genotyping by MALDI-TOF mass spectrometry, for evaluation of minor histocompatibility antigens in patients submitted to allogeneic stem cell transplantation from HLA-matched related and unrelated donor.Hematology reports · 2017Article
- Oncogenic function of the homeobox A13-long noncoding RNA HOTTIP-insulin growth factor-binding protein 3 axis in human gastric cancer.Oncotarget · 2016Article
- Telomerase reverse transcriptase methylation predicts lymph node metastasis and prognosis in patients with gastric cancer.OncoTargets and therapy · 2016Article
- MALDI-TOF Mass Array Analysis of Nell-1 Promoter Methylation Patterns in Human Gastric Cancer.BioMed research international · 2015Article
- Article
- Human CD1a deficiency is common and genetically regulated.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Application of mass spectrometry to molecular diagnostics of viral infections.Expert review of molecular diagnostics · 2013Review
- Application of maldi-tof mass spectrometry in clinical virology: a review.The open virology journal · 2013Article
- [Mass spectrometry in the clinical microbiology laboratory].Enfermedades infecciosas y microbiologia clinica · 2012Review
- Two combinatorial optimization problems for SNP discovery using base-specific cleavage and mass spectrometry.BMC systems biology · 2012Article
- Chronic maternal protein deprivation in mice is associated with overexpression of the cohesin-mediator complex in liver of their offspring.The Journal of nutrition · 2011Article
- Multilocus sequence typing of Streptococcus pneumoniae by use of mass spectrometry.Journal of clinical microbiology · 2011Article
- Investigation of DNA damage response and apoptotic gene methylation pattern in sporadic breast tumors using high throughput quantitative DNA methylation analysis technology.Molecular cancer · 2010Article
- Robust hepatitis B virus genotyping by mass spectrometry.Journal of clinical microbiology · 2010Article
- Development and characterization of an oat TILLING-population and identification of mutations in lignin and beta-glucan biosynthesis genes.BMC plant biology · 2010Article
- High-throughput analysis of candidate imprinted genes and allele-specific gene expression in the human term placenta.BMC genetics · 2010Article
- Mass spectrometry tools for the classification and identification of bacteria.Nature reviews. Microbiology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a comparative sequencing strategy that is based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analyses of complete base-specific cleavage reactions of a target sequence. The target is converted to a DNA/RNA mosaic structure after PCR amplification using in vitro transcription. Cleavage with defined specificity is achieved by ribonucleases. The set of cleavage products is subjected to mass spectrometry without prior fractionation. The presented resequencing assay is particularly useful for single-nucleotide polymorphism (SNP) discovery. The combination of mass spectra from four complementary cleavage reactions detects approximately 98% of all possible homozygous and heterozygous SNPs in target sequences with a length of up to 500 bases. In general, both the identity and location of the sequence variation are determined. This was exemplified by the discovery of SNPs in the human gene coding for the cholesteryl ester transfer protein using a panel of 96 genomic DNAs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.