ArticleGenome research2004
Identification of antibiotic stress-inducible promoters: a systematic approach to novel pathway-specific reporter assays for antibacterial drug discovery.
Article in Genome research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Construction of a novel reporter strain indicating inhibition of cell wall biosynthesis.Applied microbiology and biotechnology · 2026Article
- Exploring FabH inhibitors for antimicrobial therapy: medicinal chemistry, synthetic approaches, and SAR evaluation.Molecular diversity · 2026Review
- Microbial Genomics: Innovative Targets and Mechanisms.Antibiotics (Basel, Switzerland) · 2023Review
- Discovery, isolation, heterologous expression and mode-of-action studies of the antibiotic polyketide tatiomicin from Amycolatopsis sp. DEM30355.Scientific reports · 2022Article
- The pursuit of mechanism of action: uncovering drug complexity in TB drug discovery.RSC chemical biology · 2021Article
- A Small Molecule Inhibitor of CTP Synthetase Identified by Differential Activity on aFrontiers in microbiology · 2020Article
- The challenge of converting Gram-positive-only compounds into broad-spectrum antibiotics.Annals of the New York Academy of Sciences · 2019Review
- Production of Ramoplanin and Ramoplanin Analogs by Actinomycetes.Frontiers in microbiology · 2017Article
- Bioluminescent Reporters for Rapid Mechanism of Action Assessment in Tuberculosis Drug Discovery.Antimicrobial agents and chemotherapy · 2016Article
- Article
- Discovering new bioactive molecules from microbial sources.Microbial biotechnology · 2014Review
- Double recombinant Mycobacterium bovis BCG strain for screening of primary and rationale-based antimycobacterial compounds.Antimicrobial agents and chemotherapy · 2014Article
- Time-resolved transcriptome analysis of Bacillus subtilis responding to valine, glutamate, and glutamine.PloS one · 2009Article
- Novel whole-cell antibiotic biosensors for compound discovery.Applied and environmental microbiology · 2007Article
- Novel approach to mapping of resistance mutations in whole genomes by using restriction enzyme modulation of transformation efficiency.Antimicrobial agents and chemotherapy · 2005Article
- Discovering the mechanism of action of novel antibacterial agents through transcriptional profiling of conditional mutants.Antimicrobial agents and chemotherapy · 2005Article
- Differential gene expression to investigate the effect of (5Z)-4-bromo- 5-(bromomethylene)-3-butyl-2(5H)-furanone on Bacillus subtilis.Applied and environmental microbiology · 2004Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As present antibiotics therapy becomes increasingly ineffectual, new technologies are required to identify and develop novel classes of antibacterial agents. An attractive alternative to the classical target-based approach is the use of promoter-inducible reporter assays for high-throughput screening. The wide usage of these assays is, however, limited by the small number of specifically responding promoters that are known at present. This work describes a novel approach for identifying genetic regulators that are suitable for the design of pathway-specific assays. The basis for the proposed strategy is a large set of antibiotics-triggered expression profiles ("Reference Compendium"). Pattern recognition algorithms applied to the expression data pinpoint the relevant transcription-factor-binding sites in whole-genome sequences. Using this technique, we constructed a fatty-acid-pathway-specific reporter assay that is based on a novel stress-inducible promoter. In a proof-of-principle experiment, this assay was shown to enable screening for new small-molecule inhibitors of bacterial growth.
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