ArticleNucleic acids research2004
Dual-regulated expression of C/EBP-alpha and BMP-2 enables differential differentiation of C2C12 cells into adipocytes and osteoblasts.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 71 citations in OpenAlex.
- Effect of ciglitazone on adipogenic transdifferentiation of bovine skeletal muscle satellite cells.Journal of animal science and technology · 2021Article
- FGF10 and Lipofibroblasts in Lung Homeostasis and Disease: Insights Gained From the Adipocytes.Frontiers in cell and developmental biology · 2021Review
- Oleic acid in the absence of a PPARγ agonist increases adipogenic gene expression in bovine muscle satellite cells1.Journal of animal science · 2019Article
- MORC2 regulates C/EBPα-mediated cell differentiation via sumoylation.Cell death and differentiation · 2019Article
- Discovery of a Small Molecule Promoting Mouse and Human Osteoblast Differentiation via Activation of p38 MAPK-β.Cell chemical biology · 2019Article
- Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Lipogenesis in myoblasts and its regulation of CTRP6 by AdipoR1/Erk/PPARγ signaling pathway.Acta biochimica et biophysica Sinica · 2016Article
- Novel Wnt Regulator NEL-Like Molecule-1 Antagonizes Adipogenesis and Augments Osteogenesis Induced by Bone Morphogenetic Protein 2.The American journal of pathology · 2016Article
- Meeting the meat: delineating the molecular machinery of muscle development.Journal of animal science and technology · 2016Review
- Brown fat determination and development from muscle precursor cells by novel action of bone morphogenetic protein 6.PloS one · 2014Article
- The effect of troglitazone on lipid accumulation and related gene expression in Hanwoo muscle satellite cell.Journal of physiology and biochemistry · 2013Article
- Developing peptide-based multivalent antagonists of proliferating cell nuclear antigen and a fluorescence-based PCNA binding assay.Analytical biochemistry · 2012Article
- Replicative senescence of human bone marrow and umbilical cord derived mesenchymal stem cells and their differentiation to adipocytes and osteoblasts.Molecular biology reports · 2011Article
- Effects of gender-specific adult bovine serum on myogenic satellite cell proliferation, differentiation and lipid accumulation.In vitro cellular & developmental biology. Animal · 2011Article
- Article
- Notch and Wnt signaling, physiological stimuli and postnatal myogenesis.International journal of biological sciences · 2010Review
- The insulin-like growth factor-1 binding protein acid-labile subunit alters mesenchymal stromal cell fate.The Journal of biological chemistry · 2010Article
- The role of CCAAT/enhancer binding protein (C/EBP)-alpha in osteogenesis of C3H10T1/2 cells induced by BMP-2.Journal of cellular and molecular medicine · 2009Article
- A comprehensive analysis of the dual roles of BMPs in regulating adipogenic and osteogenic differentiation of mesenchymal progenitor cells.Stem cells and development · 2009Article
- FMIP controls the adipocyte lineage commitment of C2C12 cells by downmodulation of C/EBP alpha.Oncogene · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CCAAT/enhancer-binding proteins (C/EBPs) as well as bone morphogenic proteins (BMPs) play essential roles in mammalian cell differentiation in shaping adipogenic and osteoblastic lineages in particular. Recent evidence suggested that adipocytes and osteoblasts share a common mesenchymal precursor cell phenotype. Yet, the molecular details underlying the decision of adipocyte versus osteoblast differentiation as well as the involvement of C/EBPs and BMPs remains elusive. We have engineered C2C12 cells for dual-regulated expression of human C/EBP-alpha and BMP-2 to enable independent transcription control of both differentiation factors using clinically licensed antibiotics of the streptogramin (pristinamycin) and tetracycline (tetracycline) classes. Differential as well as coordinated expression of C/EBP-alpha and BMP-2 revealed that (i) C/EBP-alpha may differentiate C2C12 myoblasts into adipocytes as well as osteoblasts, (ii) BMP-2 prevents myotube differentiation, (iii) is incompetent in differentiating C2C12 into osteoblasts and (iv) even decreases C/EBP-alpha's osteoblast-specific differentiation potential but (v) cooperates with C/EBP-alpha on adipocyte differentiation, (vi) osteoblast formation occurs at low C/EBP-alpha levels while adipocyte-specific differentiation requires maximum C/EBP-alpha expression and that (vii) BMP-2 may bias the C/EBP-alpha-mediated adipocyte versus osteoblast differentiation switch towards fat cell formation. Dual-regulated expression technology enabled precise insight into combinatorial effects of two key differentiation factors involved in adipocyte/osteoblast lineage control which could be implemented in rational reprogramming of multipotent cells into desired cell phenotypes tailored for gene therapy and tissue engineering.
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