ArticleNucleic acids research2004
Formation of DNA nanoparticles in the presence of novel polyamine analogues: a laser light scattering and atomic force microscopic study.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 74 citations in OpenAlex.
- Biodegradable Polymers for Gene Delivery.Molecules (Basel, Switzerland) · 2019Review
- In Situ Visualization of the Local Photothermal Effect Produced on α-Cyclodextrin Inclusion Compound Associated with Gold Nanoparticles.Nanoscale research letters · 2016Article
- DNA Interaction Studies of Selected Polyamine Conjugates.International journal of molecular sciences · 2016Article
- Synthetic tumor networks for screening drug delivery systems.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- The structure-forming role of magnesium pyrophosphate in the formation of DNA-containing microparticles during PCR.Doklady. Biochemistry and biophysics · 2015Article
- Morphology, structure and function characterization of PEI modified magnetic nanoparticles gene delivery system.PloS one · 2014Article
- A universal description for the experimental behavior of salt-(in)dependent oligocation-induced DNA condensation.Nucleic acids research · 2012Article
- Characterization of condensed plasmid DNA models for studying the direct effect of ionizing radiation.Biophysical chemistry · 2010Article
- A universal description for the experimental behavior of salt-(in)dependent oligocation-induced DNA condensation.Nucleic acids research · 2009Article
- The triple helix: 50 years later, the outcome.Nucleic acids research · 2008Review
- Peptide-functionalized poly(ethylene glycol) star polymers: DNA delivery vehicles with multivalent molecular architecture.Bioconjugate chemistry · 2008Article
- Mechanistic differences in DNA nanoparticle formation in the presence of oligolysines and poly-L-lysine.Biomacromolecules · 2007Article
- In vitro non-viral gene delivery with nanofibrous scaffolds.Nucleic acids research · 2005Article
- Condensation of oligonucleotides assembled into nicked and gapped duplexes: potential structures for oligonucleotide delivery.Nucleic acids research · 2005Article
- Enhanced cellular uptake of a triplex-forming oligonucleotide by nanoparticle formation in the presence of polypropylenimine dendrimers.Nucleic acids research · 2004Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We synthesized a pentamine (3-3-3-3) and two hexamine (3-3-3-3-3 and 3-4-3-4-3) analogues of the natural polyamine, spermine (3-4-3) and studied their effectiveness in condensing pGL3 plasmid DNA, using light scattering and atomic force microscopic (AFM) techniques. The midpoint concentration of the polyamines on pGL3 condensation (EC50) was 11.3, 10.6, 1.5, 0.49 and 0.52 micro M, respectively, for 3-4-3, norspermine (3-3-3), 3-3-3-3, 3-3-3-3-3 and 3-4-3-4-3 in 10 mM Na cacodylate buffer. Dynamic laser light scattering study showed a decrease in hydrodynamic radii of plasmid DNA particles as the number of positive charges on the polyamines increased. AFM data showed the presence of toroids with outer diameter of 117-191 nm for different polyamines, and a mean height of 2.61 +/- 0.77 nm. AFM results also revealed the presence of intermediate structures, including those showing circumferential winding of DNA to toroids. The dependence of the EC50 on Na+ concentration suggests different modes of binding of spermine and its higher valent analogues with DNA. Our results show a 20-fold increase in the efficacy of hexamines for DNA condensation compared to spermine, and provide new insights into the mechanism(s) of DNA nanoparticle formation. These studies might help to develop novel nonviral gene delivery vehicles.
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