ArticleNucleic acids research2004
Role of intrinsic DNA binding specificity in defining target genes of the mammalian transcription factor PDX1.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
- PDX1 phosphorylation at S61 by mTORC1 links nutrient signaling to β cell function and metabolic disease.Cell reports · 2026Article
- Effect of transcription factor MEOX on insulin gene expression in glucagon-like peptide 1-secreting cells.In vitro cellular & developmental biology. Animal · 2024Article
- The chromatin accessibility dynamics during cell fate specifications in zebrafish early embryogenesis.Nucleic acids research · 2024Article
- The Interaction of Natural Selection and GC Skew May Drive the Fast Evolution of a Sand Rat Homeobox Gene.Molecular biology and evolution · 2019Article
- Transcription factor Ets-1 links glucotoxicity to pancreatic beta cell dysfunction through inhibiting PDX-1 expression in rodent models.Diabetologia · 2016Article
- Role of transcription factors in the transdifferentiation of pancreatic islet cells.Journal of molecular endocrinology · 2015Review
- Establishment of a novel, eco-friendly transgenic pig model using porcine pancreatic amylase promoter-driven fungal cellulase transgenes.Transgenic research · 2015Article
- Differentiation of Human Mesenchymal Stem Cells into Insulin Producing Cells by Using A Lentiviral Vector Carrying PDX1.Cell journal · 2015Article
- Thermodynamic and structural determinants of differential Pdx1 binding to elements from the insulin and IAPP promoters.Journal of molecular biology · 2013Article
- Binding polymorphism in the DNA bound state of the Pdx1 homeodomain.PLoS computational biology · 2013Article
- Variation in homeodomain DNA binding revealed by high-resolution analysis of sequence preferences.Cell · 2008Article
- Transcriptional regulation of the distal promoter of the rat pyruvate carboxylase gene by hepatocyte nuclear factor 3beta/Foxa2 and upstream stimulatory factors in insulinoma cells.The Biochemical journal · 2007Article
- A feat of metabolic proportions: Pdx1 orchestrates islet development and function in the maintenance of glucose homeostasis.Molecular genetics and metabolismReview
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PDX1 is a homeodomain transcription factor essential for pancreatic development and mature beta cell function. Homeodomain proteins typically recognize short TAAT DNA motifs in vitro: this binding displays paradoxically low specificity and affinity, given the extremely high specificity of action of these proteins in vivo. To better understand how PDX1 selects target genes in vivo, we have examined the interaction of PDX1 with natural and artificial binding sites. Comparison of PDX1 binding sites in several target promoters revealed an evolutionarily conserved pattern of nucleotides flanking the TAAT core. Using competitive in vitro DNA binding assays, we defined three groups of binding sites displaying high, intermediate and low affinity. Transfection experiments revealed a striking correlation between the ability of each sequence to activate transcription in cultured beta cells, and its ability to bind PDX1 in vitro. Site selection from a pool of oligonucleotides (sequence NNNTAATNNN) revealed a non-random preference for particular nucleotides at the flanking locations, resembling natural PDX1 binding sites. Taken together, the data indicate that the intrinsic DNA binding specificity of PDX1, in particular the bases adjacent to TAAT, plays an important role in determining the spectrum of target genes.
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