ArticleJournal of bacteriology2004
The Caulobacter crescentus CgtAC protein cosediments with the free 50S ribosomal subunit.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 72 citations in OpenAlex.
- The role of the essential GTPase ObgE in regulating lipopolysaccharide synthesis in Escherichia coli.Nature communications · 2024Article
- Article
- Oryza sativa ObgC1 Acts as a Key Regulator of DNA Replication and Ribosome Biogenesis in Chloroplast Nucleoids.Rice (New York, N.Y.) · 2021Article
- Human GTPBP5 is involved in the late stage of mitoribosome large subunit assembly.Nucleic acids research · 2021Article
- Article
- RiceG3 (Bethesda, Md.) · 2018Article
- Complementation of the Mycoplasma synoviae MS-H vaccine strain with wild-type obg influencing its growth characteristics.PloS one · 2018Article
- BcMtg2 is required for multiple stress tolerance, vegetative development and virulence in Botrytis cinerea.Scientific reports · 2016Article
- Article
- The Neisseria gonorrhoeae Obg protein is an essential ribosome-associated GTPase and a potential drug target.BMC microbiology · 2015Article
- Human G-proteins, ObgH1 and Mtg1, associate with the large mitochondrial ribosome subunit and are involved in translation and assembly of respiratory complexes.Nucleic acids research · 2013Article
- Mutations in GTP binding protein Obg of Mycoplasma synoviae vaccine strain MS-H: implications in temperature-sensitivity phenotype.PloS one · 2013Article
- In vivo biochemistry in bacterial cells using FRAP: insight into the translation cycle.Biophysical journal · 2012Article
- C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation.Nucleic acids research · 2012Article
- Biochemical characterization of ribosome assembly GTPase RbgA in Bacillus subtilis.The Journal of biological chemistry · 2012Article
- The universally conserved prokaryotic GTPases.Microbiology and molecular biology reviews : MMBR · 2011Review
- An HflX-type GTPase from Sulfolobus solfataricus binds to the 50S ribosomal subunit in all nucleotide-bound states.Journal of bacteriology · 2011Article
- Biochemical and physiological characterization of the GTP-binding protein Obg of Mycobacterium tuberculosis.BMC microbiology · 2011Article
- Molecular modeling study for interaction between Bacillus subtilis Obg and Nucleotides.PloS one · 2010Article
- Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering.Molecular & cellular proteomics : MCP · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Obg family of GTPases is widely conserved and predicted to play an as-yet-unknown role in translation. Recent reports provide circumstantial evidence that both eukaryotic and prokaryotic Obg proteins are associated with the large ribosomal subunit. Here we provide direct evidence that the Caulobacter crescentus CgtA(C) protein is associated with the free large (50S) ribosomal subunit but not with 70S monosomes or with translating ribosomes. In contrast to the Bacillus subtilis and Escherichia coli proteins, CgtA(C) does not fractionate in a large complex by gel filtration, indicating a moderately weak association with the 50S subunit. Moreover, binding of CgtA(C) to the 50S particle is sensitive to salt concentration and buffer composition but not guanine nucleotide occupancy of CgtA(C). Assays of epitope-tagged wild-type and mutant variants of CgtA(C) indicate that the C terminus of CgtA(C) is critical for 50S association. Interestingly, the addition of a C-terminal epitope tag also affected the ability of various cgtA(C) alleles to function in vivo. Depletion of CgtA(C) led to perturbations in the polysome profile, raising the possibility that CgtA(C) is involved in ribosome assembly or stability.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.