ArticleGenes & development2004
hRIP, a cellular cofactor for Rev function, promotes release of HIV RNAs from the perinuclear region.
Article in Genes & development, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 81 citations in OpenAlex.
- Development and characterization of a double-fluorescent HIV-1 reporter cellular model to tackle the Rev-dependent export pathway.Microbiology spectrum · 2025Article
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- Crucial Genes in Aortic Dissection Identified by Weighted Gene Coexpression Network Analysis.Journal of immunology research · 2022Article
- A plant-specific syntaxin-6 protein contributes to the intracytoplasmic route for the begomovirus CabLCV.Plant physiology · 2021Article
- The importance of virion-incorporated cellular RNA-Binding Proteins in viral particle assembly and infectivity.Seminars in cell & developmental biology · 2021Review
- Strength in Diversity: Nuclear Export of Viral RNAs.Viruses · 2020Review
- A Begomovirus Nuclear Shuttle Protein-Interacting Immune Hub: Hijacking Host Transport Activities and Suppressing Incompatible Functions.Frontiers in plant science · 2020Review
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- WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins.PloS one · 2017Article
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- Profiling of alternative polyadenylation sites in luminal B breast cancer using the SAPAS method.International journal of molecular medicine · 2015Article
- Cooperativity among Rev-associated nuclear export signals regulates HIV-1 gene expression and is a determinant of virus species tropism.Journal of virology · 2014Article
- Human protein Staufen-2 promotes HIV-1 proliferation by positively regulating RNA export activity of viral protein Rev.Retrovirology · 2014Article
- ITGB5 and AGFG1 variants are associated with severity of airway responsiveness.BMC medical genetics · 2013Article
- A viral-human interactome based on structural motif-domain interactions captures the human infectome.PloS one · 2013Article
- Article
- Detection of viral RNA by fluorescence in situ hybridization (FISH).Journal of visualized experiments : JoVE · 2012Article
- Formation of trans-activation competent HIV-1 Rev:RRE complexes requires the recruitment of multiple protein activation domains.PloS one · 2012Article
- Role of Nucleocytoplasmic RNA Transport during the Life Cycle of Retroviruses.Frontiers in microbiology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Human immunodeficiency virus Rev facilitates the cytoplasmic accumulation of viral RNAs that contain a Rev binding site. A human Rev-interacting protein (hRIP) was originally identified based on its ability to interact with the Rev nuclear export signal (NES) in yeast two-hybrid assays. To date, however, the function of hRIP and a role for hRIP in Rev-directed RNA export have remained elusive. Here we ablate hRIP activity with a dominant-negative mutant or RNA interference and analyze Rev function by RNA in situ hybridization. We find, unexpectedly, that in the absence of functional hRIP, Rev-directed RNAs mislocalize and aberrantly accumulate at the nuclear periphery, where hRIP is localized. In contrast, in the absence of Rev or the Rev cofactor CRM1, Rev-directed RNAs remain nuclear. We further show that the RNA mislocalization pattern resulting from loss of hRIP activity is highly specific to Rev function: the intracellular distribution of cellular poly(A)(+) mRNA, nuclear proteins, and, most important, NES-containing proteins, are unaffected. Thus, hRIP is an essential cellular Rev cofactor, which acts at a previously unanticipated step in HIV-1 RNA export: movement of RNAs from the nuclear periphery to the cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.